Expression and clinical significance of CD85d in acute myeloid leukemia
- Author:
RAO Ruo
;
WANG Shuwen
;
WU Lifang
;
WANG Zhaoliang
- Publication Type:Journal Article
- Keywords:
CD85d;
acute myeloid leukemia;
flow cytometry
- From:
China Tropical Medicine
2024;24(8):978-
- CountryChina
- Language:Chinese
-
Abstract:
Abstract: Objective This study is assigned to explore the expression of CD85d in acute myeloid leukemia and its clinical significance in the diagnosis of monocyte associated acute myeloid leukemia. Methods We used flow cytometry by CD45/SSC to analyze the expression of CD85d, CD14, CD64 in 46 monocyte associated acute myeloid leukemia (M-AML) and 60 non monocyte associated acute myeloid leukemia (NM-AML) patients admitted to the Hematology Department of Hainan General Hospital from March 2022 to December 2023. Results Both normal granulocytes and normal monocytes express CD85d, and monocytes express fluorescence with stronger intensity than granulocytes. CD85d is not expressed in normal lymphocytes.The positive expression rate of CD85d in M-AML was 80.4%（37/46）, which was significantly higher than that in NM-AML 3.3%（2/60), and the difference was statistically significant (P<0.01).The sensitivity of diagnosing M-AML was ranked from high to low as CD64 (87.0%)>CD85d (80.4%)>CD14 (34.8%). The specificity of diagnosing M-AML was ranked from high to low as with CD14 (100%)>CD85d (96.7%)>CD64 (56.7%).The sensitivity and specificity of CD85d combined with CD64 in diagnosing M-AML were 89.1% and 56.7%.There was no statistically significant difference between the detection rate of abnormal karyotypes (53.8%), positive rate of the fusion genes(26.9%), the CR rate (78.3%) in CD85d+ AML and that in CD85d- AML (66.0%, 50.0%, 70.0%) (P>0.05).Conclusion CD85d can be used as a new high sensitivity and specific surface marker for immature monocytes, which is helpful to improve the detection rate of M-AML and differential diagnosis with NM-AML subtypes.
- Full text:202511071102583949616.Expression and clinical significance of CD85d in acute myeloid leukemia.pdf
