Study on HPLC fingerprint and quantitative analysis of multi-components by single-marker content determination method for Shechuan naolitong granules
- VernacularTitle:麝川脑立通颗粒的HPLC指纹图谱及一测多评含量测定方法研究
- Author:
Xiaoyan ZHANG
1
;
Kairu DING
2
;
Hong ZHANG
1
;
Wenbing ZHI
1
;
Shengnan JIANG
1
;
Zongren XU
1
;
Ni CUI
3
;
Xiangfeng WEI
3
;
Yang LIU
1
Author Information
1. Institute of Chinese Materia Medica，Shaanxi Academy of Traditional Chinese Medicine （Shaanxi Hospital of TCM），Xi’an 710003，China
2. College of Life Science，Northwest University，Xi’an 710069，China
3. Medicine Preparation Center，Xi’an TCM Hospital of Encephalopathy，Xi’an 710032，China;Xi’an TCM Preparation Engineering Technology Research Center，Xi’an 710032，China
- Publication Type:Journal Article
- Keywords:
Shechuan naolitong granules;
fingerprint;
quantitative analysis of multi-components by single-marker method
- From:
China Pharmacy
2025;36(19):2409-2414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide a reference for optimizing and promoting the quality standards of Shechuan naolitong granules. METHODS Fifteen batches of Shechuan naolitong granules were used as samples to establish HPLC fingerprints using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine （2012 edition）. Similarity evaluation and common peak identification were performed， and orthogonal partial least squares discriminant analysis （OPLS-DA） was used to assess quality differences among different batches and to screen quality differential components. Using salvianolic acid B（SAB） as the internal reference， quantitative analysis of multi-components by single-marker （QAMS） was developed to simultaneously determine geniposidic acid （GA）， chlorogenic acid （CA）， vaccarin （VA）， ferulic acid （FA） and senkyunolide I （SI）. The results were compared with those obtained by the external standard method. RESULTS A total of 13 common peaks were identified in the HPLC fingerprints of 15 batches of samples， and the similarities of the spectra were all above 0.96. Seven chromatographic peaks were identified as GA （peak 3）， CA （peak 6）， VA （peak 8）， FA （peak 9）， SI （peak 11）， SAB（peak 12） and TA（peak 13）. OPLS-DA indicated that the differential quality markers among 15 batches were peaks 5， 11 （SI）， and 12 （SAB）.Using SAB as the internal reference， the relative correction factors for GA， CA， VA， FA and SI were calculated as 1.058 4， 0.594 3， 0.643 3， 0.342 7 and 0.262 8， respectively. The mean content of GA， CA， VA， FA， SI and SAB across the 15 batches of samples were 0.155 0， 0.085 4， 0.140 3， 0.071 8， 0.072 7， 1.276 3 mg/g， respectively， showing no significant difference compared with the ESM （P＞0.05）. CONCLUSIONS The established HPLC fingerprint and QAMS are simple， efficient and economical， providing a reference for the quality control and further development of Shechuan naolitong granules.
