Etiology and DNA genotypes of human adenovirus (HAdV) in 5566 children with acute lower respiratory infection
10.3969/j.issn.1006-2483.2025.05.018
- VernacularTitle:5566例急性下呼吸道感染患儿人腺病毒（HAdV）病原及其DNA基因型分析
- Author:
Qiaowei REN
1
;
Yue CHEN
1
Author Information
1. Department of Laboratory Medicine , Yan'an People's Hospital, Yan'an , Shaanxi 716000 , China
- Publication Type:Journal Article
- Keywords:
Acute lower respiratory infection;
Human adenovirus;
Etiology;
Genotype;
Clinical characteristic
- From:
Journal of Public Health and Preventive Medicine
2025;36(5):80-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze etiology and DNA genotypes of human adenovirus (HAdV) in 5566 children with acute lower respiratory infection (ALRI). Methods A total of 5 566 children with ALRI admitted to the hospital were between October 2023 and October 2024, and their nasopharyngeal aspirates were collected. HAdV and the other etiology were detected by real-time fluorescent quantitative polymerase chain reaction (qPCR). The viruses isolation in HAdV positive samples was performed, and DNA genotypes of the isolated viruses were detected by PCR amplification. The clinical characteristics of children with ALRI-HAdV and different DNA genotypes were analyzed. Results Among the 5 566 children with ALRI, positive rate of HAdV was 27.56% (1534/5566). The positive rate was the highest in those with age of 3-6 years (57.51%). There was HAdV infection throughout the year, and the incidence was the highest in summer (41.39%). Among 1534 children with HAdV positive, there were 976 cases with pneumonia and 558 cases with bronchitis. The common clinical manifestations were fever, cough, dry and sore throat, and common complications were respiratory failure and myocardial injury. There were 1201 children with successful classifications, proportions of HAV-B, HAV-C and HAV-E were 53.04%, 42.96% and 4.00%, respectively. The detection rate of HAV-B3 in subgroup B was the highest (34.05%), followed by HaV-C1 in subgroup C (19.48%). There was no difference in distribution of HAdV classifications among children with different genders and age (P>0.05). However, there were differences in distribution of HAdV classifications among admission children in different seasons (P<0.05). Among 1201 children, there were 457 cases with single HAdV infection and 744 cases with mixed infection, and incidence of mixed infection (Mycoplasma pneumoniae infection + influenza virus infection) was higher. Conclusions The incidence of HAdV infection is the highest in ALRI children with age of 3-6 years, and the onset is mainly in summer. The main infection type is Mycoplasma pneumoniae infection + influenza virus infection, main manifestations include fever, cough, dry throat and sore throat, and main genotypes of HAdV infection include HAdV-B3 and HAdV-C1.
