Reproductive toxicity of clothianidin on two generations of Wistar rats
10.3969/j.issn.1006-2483.2025.05.004
- VernacularTitle:噻虫胺原药对Wistar大鼠两代繁殖毒性研究
- Author:
Yinghua LIU
1
;
Qinghua ZHOU
1
;
Shufei LI
1
;
Miao ZHAO
1
;
Dianming ZHOU
1
;
Zhiyong QIAN
1
Author Information
1. Tianjin Centers for Disease Control and Prevention , Tianjin 300011 , China
- Publication Type:Journal Article
- Keywords:
Clothianidin;
Reproductive toxicity;
NOAEL
- From:
Journal of Public Health and Preventive Medicine
2025;36(5):18-22
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the reproductive and developmental effects of Clothianidin in rats. Methods Clothianidin was administrated by diet to both parental and first filial (F 1) generations of rats at the dosages of 0, 30.51, 110.84 and 304.26 mg/(kg·d) in females, and 0, 26.45, 92.69 and 279.42 mg/(kg·d) in males. Clothianidin was administered through diet to male and female rats for 8 weeks before mating. Clothianidin was administered to female rats in the parental and F1 generations during mating, gestation and lactation periods. During the test, toxicity performance was observed, reproduction index was calculated, and pathological examination was carried out. Results The body weights of rats in the parent and F1 generations in the high-dose group were lower than those in the control group during pre-mating exposure and at various time points during pregnancy and lactation (P<0.05). The pregnancy rates of parental and F1 generations in the high-dose group were lower than those of the control group (48.57% vs 71.43%, 45.71% vs 80.00%, P＜0. 05). Sperm concentration and sperm motility of the parental generation were lower than those of the control group [(42.55±12.87) vs (53.84±7.65) ×106/ml, (58.94±10.59) vs (65.59±6.03), （P＜0.05）]. Sperm concentration and sperm motility of the F1 generation were lower than those of the control group [(41.64±12.42) vs (53.09±9.48), (55.13±9.19) vs (64.53±6.31), (P＜0.05). Conclusion Exposure to clothianidin has reproductive toxicity to Wistar rats, and the no-observed adverse effect level (NOAEL) in the two-generation reproductive toxicity test is 92.69 mg/kg·BW for males and 110.84 mg/kg·BW for females in Wistar rats.
