Circular RNA hsa_circ_0081621 promotes malignant biological behavior of human laryngeal squamous cell carcinoma cells

LIU Shenghui1,2; AI Xueying1,2; XU Yuru1; LI Shuqi1,2; LIU Meng1; HU Guobin1; SHAN Baoen2,3

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Circular RNA hsa_circ_0081621 promotes malignant biological behavior of human laryngeal squamous cell carcinoma cells

VernacularTitle:环状RNA hsa_circ_0081621促进人喉鳞状细胞癌细胞的恶性生物学行为
Author: LIU Shenghui1,2 ^{1
,

2
,

3} ; AI Xueying1,2 ^{1
,

2
,

3} ; XU Yuru1 ^{1
,

2
,

3} ; LI Shuqi1,2 ^{1
,

2
,

3} ; LIU Meng1 ^{1
,

2
,

3} ; HU Guobin1 ^{1
,

2
,

3} ; SHAN Baoen2,3 ^{1
,

2
,

3}
Author Information

1. Department of Otolaryngology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China;
2. Department of Research Center, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China;
3. Tumor Research Institute, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei, China
Publication Type:Journal Article
Keywords: 喉鳞状细胞癌；AMC-HN-8细胞；TU177细胞；环状RNA； hsa_circ_0081621；增殖；迁移；侵袭
From: Chinese Journal of Cancer Biotherapy 2025;32(6):579-586
CountryChina
Language:Chinese
Abstract: [摘要] 目的：探究环状RNA hsa_circ_0081621对人喉鳞状细胞癌AMC-HN-8和TU177细胞恶性生物学行为的影响。方法：常规培养AMC-HN-8和TU177细胞，用转染试剂将si-NC、si-hsa_circ_0081621、空载体（vector）和hsa_circ_0081621过表达载体（hsa_circ_0081621-OE）转染至AMC-HN-8和TU177细胞，分别记作si-NC、si-hsa_circ_0081621、vector和hsa_circ_0081621-OE组。用CCK-8法、克隆形成实验、划痕愈合实验和Transwell小室实验分别检测敲减或过表达hsa_circ_0081621对AMC-HN-8和TU177细胞增殖、迁移和侵袭能力的影响。结果：在AMC-HN-8和TU177中成功地敲减或过表达了hsa_circ_0081621；敲减或过表达hsa_circ_0081621能显著抑制或促进AMC-HN-8和TU177细胞的增殖、迁移和侵袭能力（P < 0.01或P < 0.001或P < 0.000 1）。结论：hsa_circ_0081621可促进人喉鳞状细胞癌AMC-HN-8和TU177细胞的恶性生物学行为。
Full text:202507150901013153720250603.pdf