Serological baseline, antibody stability and efficacy of different types of avian influenza (H5) vaccines
- Author:
Mohammed Reza ROUYGARI
1
;
Mansour MAYAHI
;
Mehdi Vasfi MARANDY
;
Zahra BOROOMAND
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2025;26(1):e4-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Objective:To establish a serological baseline, assess antibody stability, and compare the efficacy of three HPAI (H5) vaccines.
Methods:We analyzed over 9,000 blood samples and 6,420 swabs from approximately 1.5 million birds up to 64 weeks old. HI (β, α), RT-PCR, and SN tests were conducted, with statistical analysis performed using two-way ANOVA.
Results:The serological baseline (GM titer) using H5N8 antigens from A/Chicken/Iran/162/ 2016 varied. The Re6+Re8 vaccine produced higher and more stable HI β titers than the H5N3 and baculovirus vaccines. Serum HI α neutralization ability was similar for Re6+Re8 and H5N3 vaccines, both 100 times greater than the baculovirus vaccine. Neutralization indices for H5N3, Re6+Re8, and baculovirus vaccines were 4.7, 4.5, and 4.2 (log2), respectively.
Conclusions:and Relevance: After two vaccinations, Re6+Re8 exhibited the most stable HI β antibody response, while H5N3 had the highest neutralization index, surpassing Re6+Re8 by 0.2 and the baculovirus vaccine by 0.5. These findings highlight discrepancies between HI β and SN test results, with SN being a stronger indicator of protective titers due to its in vivo methodology, compared to the in vitro HI assay.
