Effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolites in liver cirrhosis with minimal hepatic encephalopathy： A study based on metabolomics

Tingting JIANG; Qun ZHANG; Xianbo WANG; Yuyong JIANG; Xiaoli LIU; Hao YU; Zhiyun YANG

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Effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolites in liver cirrhosis with minimal hepatic encephalopathy： A study based on metabolomics

VernacularTitle:基于代谢组学探讨益气养阴解毒化瘀方对肝硬化轻微型肝性脑病肠道代谢物的影响
Author: Tingting JIANG ¹ ; Qun ZHANG ¹ ; Xianbo WANG ¹ ; Yuyong JIANG ¹ ; Xiaoli LIU ¹ ; Hao YU ¹ ; Zhiyun YANG ¹
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1. Department of Integrated Traditional Chinese and Western Medicine， Beijing Ditan Hospital， Capital Medical University， Laboratory for Clinical Medicine， Capital Medical University， Beijing 100015， China
Publication Type:Journal Article
Keywords: Liver Cirrhosis; Hepatic Encephalopathy; Yiqi Yangyin Jiedu Huayu Prescription; Traditional Chinese Medicine Therapy; Metabolomics
From: Journal of Clinical Hepatology 2025;41(3):469-477
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the effect of Yiqi Yangyin Jiedu Huayu prescription on minimal hepatic encephalopathy in liver cirrhosis based on intestinal metabolomics. MethodsA total of 11 patients with liver cirrhosis who were hospitalized in Beijing Ditan Hospital， Capital Medical University， from March to May 2024， and were diagnosed with MHE based on psychometric hepatic encephalopathy score were enrolled as subjects， and 11 healthy family members of the patients were enrolled as control group. Fecal samples were collected for metabolomics analysis from the control group and the patients with MHE before and after treatment with Yiqi Yangyin Jiedu Huayu prescription， and a population cohort study was conducted to investigate the effect of Yiqi Yangyin Jiedu Huayu prescription on intestinal metabolism of patients with MHE. The Fisher’s exact test was used for categorical data between two groups； the independent samples t-test was used for comparison of normally distributed continuous data between two groups， the paired t-test was used for comparision before and after treatment within the same group， and the Wilcoxon rank-sum test was used for comparison of non-normally distributed continuous data between two groups. ResultsA total of 29 differentially expressed metabolites were detected between the MHE group and the control group， mainly amino acids， organic acids， organic amines， carbohydrates， fatty acids， and vitamins， and there were 12 upregulated metabolites and 17 downregulated metabolites in the MHE group， which were mainly enriched in the metabolic pathways of ornithine， branched-chain amino acid， and aromatic amino acid. After the treatment with Yiqi Yangyin Jiedu Huayu prescription， 80 differentially expressed metabolites were detected in the patients with MHE， mainly carbohydrates， organic acids， and amino acids， and there were 56 upregulated metabolites and 24 downregulated metabolites， which were mainly enriched in the metabolic pathways of ornithine， branched-chain amino acid， and aromatic amino acid. ConclusionYiqi Yangyin Jiedu Huayu prescription can exert a therapeutic effect on patients with MHE by regulating intestinal metabolism.