Three new gallic acid sugaresters from Elaeagnus oxycarpa Schlechtend leaves and their antioxidant and tyrosinase inhibitory activities
10.16438/j.0513-4870.2024-0897
- VernacularTitle:尖果沙枣叶中3个新的没食子酸糖酯及其抗氧化活性与酪氨酸酶抑制活性
- Author:
Feng-zhen CUI
1
;
Jian-hong FU
1
;
Guo-yan XU
1
;
AYEKABAYR·EKBAYR
1
;
Chang-da MA
1
Author Information
1. Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, Urumqi 830017, China; Key Laboratory of Plant Stress Biology in Arid Land, Urumqi 830017, China; College of Life Sciences, Xinjiang Normal University, Urumqi 830017, China
- Publication Type:Research Article
- Keywords:
italic>Elaeagnus oxycarpa Schlechtend leaf;
gallic acid sugarester;
structure identification;
antioxidant activity;
tyrosinase inhibitory activity
- From:
Acta Pharmaceutica Sinica
2025;60(2):434-441
- CountryChina
- Language:Chinese
-
Abstract:
Five compounds were isolated and purified from the water extract of Elaeagnus oxycarpa Schlechtend leaf by multi-dimensional reversed-phase preparative liquid chromatographic system based on the separation and enrichment model. Their structures were identified by spectral analysis such as NMR, MS, UV, IR and by comparison with literature information as 2,4(1H,3H)-pyrimidinedione (1), elaeagnussugarester B (2), elaeagnussugarester A (3), elaeagnussugarester C (4), gallic acid (5). Compounds 2-4 are new compounds, compound 1 was isolated from Elaeagnus oxycarpa Schlechtend for the first time. The antioxidant and anti-tyrosinase activities of these compounds were evaluated by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical method, the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radical method, the potassium ferricyanide reduction method and the colorimetric method with L-tyrosine as substrate. The results showed that compounds 2-5 have good antioxidant activities and inhibitory effect on tyrosinase. Compound 4 exhibited the most strong antioxidant activities, with IC50 = 3.59 ± 0.06 μmol·L-1 for DPPH free radical scavenging ability, IC50 = 10.04 ± 0.20 μmol·L-1 for ABTS free radical scavenging ability, and total reduction capacity of compound 4 was better than vitamin C respectively. Compound 3 possessed better inhibitory effect on tyrosinase with IC50 = 0.25 ± 0.06 mmol·L-1.
