Evaluation and Analysis of Animal Models of Refractory Epilepsy Reflecting Clinical Characteristics of Traditional Chinese and Western Medicine
10.19378/j.issn.1003-9783.2024.08.013
- VernacularTitle:基于中西医临床病症特点的难治性癫痫动物模型评价与分析
- Author:
Mengqi LIU
1
;
Wentao FAN
;
Qian WANG
Author Information
1. 陕西中医药大学,陕西 咸阳 712056
- Keywords:
Refractory epilepsy;
combination of disease and syndrome;
animal models;
traditional Chinese and Western medicine;
clinic
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(8):1215-1225
- CountryChina
- Language:Chinese
-
Abstract:
Objective Based on the clinical characteristics of refractory epilepsy in traditional Chinese and Western medicine,this study compares the existing animal models of refractory epilepsy,evaluates the effectiveness and limitations of the models,and explores the integration of traditional Chinese and Western medicine theory and optimization of model construction.Our aim is to provide new research directions for elucidating the mechanism and prevention of refractory epilepsy.Methods By reviewing relevant literature and summarizing the available data,we examined current refractory epilepsy animal models,focusing on their replication methods,animal strains,as well as advantages and disadvantages of animal models.Based on the Western medicine diagnostic criteria and traditional Chinese medicine syndrome differentiation criteria for refractory epilepsy,the coincidence of existing animal models and clinical symptoms were analyzed and evaluated.Results Our review of existing refractory epilepsy animal models revealed that electrical and chemical kindling are common modeling methods.The lithium-pilocarpine model and the intrahippocampal kainic acid model show high congruence with the symptom characteristics of traditional Chinese and Western medical clinical diagnosis and are widely used.Existing models mostly focus on simulating the pathological changes of refractory epilepsy,while neglecting the factors such as congenital endowment and emotional disorders,which have been emphasized in traditional Chinese medicine.Therefore,there are still certain limitations in simulating the complexity of etiology,clinical heterogeneity,and drug treatment responsiveness of refractory epilepsy.Conclusion Although replicating animal models can reflect their pathogenesis to some extent,there are still significant differences between them and the natural pathological state and clinical manifestations of the body.The existing animal models of refractory epilepsy mostly use electrical stimulation or chemical drug induction,which has certain advantages in simulating epileptic persistence and drug resistance.However,there are still limitations such as a single induction method and insufficiently complex pathological changes.In the future,efforts can be made to integrate traditional Chinese medicine syndrome differentiation and conventional electrical stimulation or chemical drug induction methods.By simulating multiple pathogenic factors,a refractory epilepsy animal model covering different traditional Chinese medicine syndrome types can be established.At the same time,multi-dimensional indicators such as behavior,electrophysiology,imaging,and histopathology can be used to construct a more comprehensive evaluation system,in order to improve the clinical relevance and translational application value of the model.During the modeling process,liver depression syndrome models can be established through methods such as tail clamping,chronic restraint,and single cage feeding with neck shackle.A model of phlegm coagulation syndrome can be established using high-fat feed feeding method.A model of qi stagnation and blood stasis syndrome can be established using tail clip and adrenaline injection.A model of essence exhaustio due to kidney deficiency syndrome can be established by combining panic induced kidney injury with fatigue swimming.It is also possible to simulate changes of yin-yang in the environment by altering lighting and temperature conditions and study the impact of environmental changes on traditional Chinese medicine animal models.The aim of this study is to provide more ideas for the treatment and research of refractory epilepsy.
