Expression of FBXW12 in pancreatic cancer tissues and its effect on the invasion and migration of pancreatic cancer cells
10.3969/j.issn.1673-9701.2024.21.018
- VernacularTitle:FBXW12在胰腺癌组织中的表达及其对胰腺癌细胞侵袭和迁移的影响
- Author:
Jinming ZHANG
1
;
Yongfeng XIE
;
Jiao NIE
;
Lin LU
;
Chao DU
Author Information
1. 锦州医科大学临沂市人民医院研究生培养基地,山东临沂 276000;临沂市人民医院消化内科,山东临沂 276000
- Keywords:
FBXW12;
Pancreatic cancer;
Prognosis;
Cell invasion;
Migration
- From:
China Modern Doctor
2024;62(21):80-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of FBXW12 in pancreatic cancer and elucidate its impact on cancer cell migration and invasion.Methods The present study utilized the GEPIA 2 database to analyze the differential expression of FBXW12 between pancreatic cancer tissues and normal tissues.Clinical data from 31 pancreatic cancer patients who underwent radical resection at Linyi People's Hospital from June 2016 to December 2022 were collected.Immunohistochemical staining was conducted to assess FBXW12 expression in both cancerous and adjacent normal tissues obtained during surgery,with subsequent follow-up for survival prognosis.Western blot and polymerase chain reaction(PCR)techniques were employed to determine FBXW12 protein expression levels in pancreatic cancer cell lines.The impact of FBXW12 on cancer cell invasion and migration was evaluated using Transwell cell invasion and scratch test.Results The results from the analysis of the GEPIA 2 database revealed a significant downregulation of FBXW12 mRNA expression in pancreatic cancer tissues compared to normal pancreatic tissues(P＜0.05).Immunohistochemical analysis demonstrated a positive expression rate of FBXW12 protein in pancreatic cancer tissues at 75.19％(23/31),whereas adjacent normal tissues exhibited a higher positive expression rate at 93.55％(29/31),indicating a statistically significant difference in FBXW12 expression between pancreatic cancer and adjacent normal tissues(P＜0.05).Additionally,the expression level of FBXW12 in pancreatic cancer tissues was found to be closely associated with lymph node metastasis(P＜0.05),patients with low expression of FBXW12 have a worse prognosis(P＜0.05).Furthermore,transfection with FBXW12 overexpression plasmid resulted in a significant decrease in the invasion and migration abilities of pancreatic cancer cells.Conclusion FBXW12 is low expressed in pancreatic cancer and is associated with the occurrence and development of pancreatic cancer.
