The effect and mechanism of a novel mikanolide derivative LY19380b on human leukemia cell HEL
10.3969/j.issn.1673-9701.2024.19.001
- VernacularTitle:薇甘菊内酯衍生物LY19380b对人白血病细胞HEL的作用及机制研究
- Author:
Qing RAO
1
;
Sheng LIU
;
Lei HUANG
;
Yanmei LI
;
Jiang ZHENG
Author Information
1. 贵州医科大学药用植物功效与利用国家重点实验室,贵州贵阳 550014;贵州医科大学药学院,贵州贵阳 550025;贵州省天然产物研究中心药理与生物活性研究所,贵州贵阳 550014
- Keywords:
Leukemia;
Mikanolide;
Apoptosis;
Mechanism
- From:
China Modern Doctor
2024;62(19):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of mikanolide derivative LY19380b in inhibiting the growth of human leukemia cell HEL.Methods Methyl thiazolyl tetrazolium(MTT)assay was used to detect the effect of compound LY19380b on the proliferation of human tumor cells,flow cytometry was used to determine cell cycle,Annexin V-FITC/PI double staining and Hoechst 33258 staining were used to determine apoptosis.Western blot was used to analyze effects of LY19380b on cell cycle and apoptosis-related proteins.Results LY19380b had anti-proliferative activity on different human tumor cells,and significantly inhibited the growth of human leukemia cell HEL.It can also induce apoptosis and affect the cell cycle of HEL.In addition,LY19380b could significantly increase the expressions of P53,BIM,BID,BAD,BAX,FLIP,P21 and Cyclin B1 proteins,while decreased the expressions of Bcl-2,p-CDC25C,p-AKT,p-STAT3 and p-ERK proteins.Conclusion By increasing the expressions of pro-apoptotic proteins BIM,BID,BAD and BAX,decreasing the expression of anti-apoptotic protein Bcl-2,LY19380b induced the apoptosis of human leukemia cell HEL,and upregulated the expressions of P21 and Cyclin B1,thus leading to cell cycle arrest in HEL.
