Characteristics of Mitochondrial Events in Synthetic Bile Acids-induced Apoptosis of Human Osteosarcoma Cells.
10.11637/kjpa.2004.17.4.351
- Author:
Gyoo Cheon KIM
1
;
Yoon HWANG
;
Jae Hyun PARK
;
Yong Suk MOON
;
Young Hyun YOO
;
Sang Hun SHIN
;
Kyung Il UM
;
Bong Soo PARK
Author Information
1. Department of Oral Anatomy, College of Dentistry, Pusan National University, Korea. parkbs@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Bile duct derivates;
Apoptosis;
MMP;
Osteosarcoma cells
- MeSH:
Apoptosis*;
Bile Ducts;
Bile*;
Cytochromes c;
Genistein;
Humans*;
Mitochondria;
Osteosarcoma*
- From:Korean Journal of Physical Anthropology
2004;17(4):351-362
- CountryRepublic of Korea
- Language:English
-
Abstract:
ABSTRACT: Apoptosis of osteosarcoma cells induced by bile duct derivates, HS-1200 was investigated with relation to mitochodria. HS-1200 induced cytochrome c and Smac/DIABLO release from mitochondria which are major factors related to apoptosis. In these apoptosis processes, release of cytochrome c was not blocked by caspase inhibitor, but release of Smac/DIABLO was blocked. BKA, a kind of PTP (permeablity transition pore) inhibitor, did not block both of them. Interestingly, the alteration of MMP was not observed by means of using JC-1 dye. Although MitoTracker, DiOC-6 and Rhodamine123 were used to confirm previous results, the decrease of MMP was not observed. In order to investigate whether this phenomenon is apoptosis-specific or cell-specific process, genistein was added to cells which usually decreased MMP. After adding genistein, MMP was not decreased, suggesting this phenomenon is cell-specific process. Conclusionally, HS-1200 induced apoptosis of osteosarcoma cells via mitochondria, cytochrome c and Smac/DIABLO were released from mitochondria without decrease of MMP. The release of Smac/DIABLO was dependent of caspase.
