Correlation between LILRB2 in monocyte subgroups of HIV-1 patients and the immune phenotype of poor immune reconstruction
10.3760/cma.j.cn112309-20231120-00138
- VernacularTitle:HIV-1感染者单核细胞亚群中人免疫球蛋白样受体亚家族B成员2与免疫重建不良免疫表型的相关性研究
- Author:
Ruojing BAI
1
;
Lili DAI
Author Information
1. 清华大学临床医学院，清华大学附属北京清华长庚医院老年医学科，北京 102218
- Keywords:
LILRB2;
Poor immune reconstruction;
HIV-1;
Monocyte subsets
- From:
Chinese Journal of Microbiology and Immunology
2024;44(11):935-942
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the co-expression of leukocyte immunoglobulin-like receptor subfamily B member 2 (LILRB2) and immune surface-related molecules in different monocyte subgroups of HIV-1 patients, and analyze the correlation between LILRB2 and poor immune reconstruction in HIV-1 infection.Methods:Men who have sex with men (MSM) with chronic HIV-1 infection presenting to the Infection Center of Beijing You′an Hospital from January 2021 to September 2022 were enrolled in this study. They were categorized into four groups: healthy controls (HCs, n=22), treatment-naive patients (TNs, n=22), immune responders (IRs, n=22), and immune non-responders (INRs, n=22). Flow cytometry was used to analyze LILRB2 expression in classical (CD14 + + CD16 -), intermediate (CD14 + + CD16 + ), and non-classical (CD14 + CD16 + + ) monocyte subsets, and the co-expression of LILRB2 with CD80, CD86, CD163, human leukocyte antigen-DR (HLA-DR), and PD-L1. Kruskal-Wallis test and Dunn′s post-hoc analysis were used for statistical analysis. Results:The expression of LILRB2 in CD14 + CD16 + + monocytes increased significantly in the INRs group as compared with that in the IRs group ( P<0.05), the TNs group ( P<0.05), and the HCs group ( P<0.001). Additionally, the co-expression of LILRB2 and CD80 on CD14 + CD16 + + monocytes increased significantly in the TNs group than that in the HCs group ( P<0.001), the IRs group ( P<0.01), and the INRs group ( P<0.001); the co-expression of LILRB2 and CD86 on CD14 + CD16 + + monocyte subsets was enhanced in the INRs group than in the HCs group ( P=0.001), the TNs group ( P<0.05), and the IRs group ( P<0.05); the co-expression of LILRB2 and CD163 on CD14 + + CD16 + monocytes represented the most significant variation among the groups, with the INRs group exhibiting significantly lower level compared to both the IRs group ( P<0.01) and the HCs group ( P<0.01). In contrast, the co-expression of HLA-DR and LILRB2 on CD14 + CD16 + + monocytes was comparable between the INRs and the TNs groups, yet significantly elevated as compared with that in the HCs group ( P<0.01, P<0.05). Conclusions:LILRB2 is associated with abnormal activation of monocytes in HIV-1-infected individuals, and the changes in its expression in monocyte subsets may have a potential correlation with the occurrence of poor immune reconstruction.
