Progress and reflection on the application of recombinase polymerase amplification technology
10.3760/cma.j.cn112309-20240529-00180
- VernacularTitle:重组酶聚合酶扩增技术的应用新进展与思考
- Author:
Ying HU
1
;
Tantao ZHANG
;
Yulin LONG
;
Qingli DONG
;
Linlin XIAO
Author Information
1. 安徽理工大学医学院，安徽 232001
- Keywords:
Recombinase polymerase amplification;
Pathogen detection;
Thermostatic amplification technology;
Instant detection
- From:
Chinese Journal of Microbiology and Immunology
2024;44(8):665-671
- CountryChina
- Language:Chinese
-
Abstract:
Recombinase polymerase amplification technology, as a new type of nucleic acid constant temperature amplification detection technology, has shown enormous application potential and development prospects in recent years. Its unique advantages such as high sensitivity, high specificity, low dependence on instruments, and the ability to integrate multiple detection modes make it widely applicable in multiple fields, especially in grassroots and on-site real-time detection. This article reviews the development history, detection principles, research and application progress of recombinant enzyme polymerase amplification technology, and looks forward to its future development, in order to provide useful references for the research and clinical application of rapid pathogen detection methods based on recombinant enzyme polymerase amplification technology.
