Comparison of in vivo pharmacokinetics of six active constituents from Shaoyao Gancao Decoction in normal and gastric ulcer rats
10.3969/j.issn.1001-1528.2024.11.006
- VernacularTitle:芍药甘草汤中6种活性成分在正常、胃溃疡大鼠体内药动学比较
- Author:
He-Rong LI
1
;
Yang JIN
;
Huan ZHANG
;
Tian-Tai WU
;
Jian WEN
;
Chao TANG
;
Xue-Yi CHENG
;
Wen LIU
Author Information
1. 贵州医科大学药学院,贵州 贵阳 550025;贵州医科大学,贵州省药物制剂重点实验室/省部共建药用植物功效与利用国家重点实验室,贵州 贵阳 550004
- Keywords:
Shaoyao Gancao Decoction;
active constituents;
gastric ulcer;
in vivo pharmacokinetics;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(11):3572-3578
- CountryChina
- Language:Chinese
-
Abstract:
AIM To compare the in vivo pharmacokinetics of paeoniflorin,paeoniflorin,liquiritin,isoliquiritin,liquiritigenin and glycyrrhizic acid from Shaoyao Gancao Decoction in normal and gastric ulcer rats.METHODS Six rats were randomly assigned into two groups,after which the 75%ethanol-induced gastric ulcer model was established,the gastric tissues were collected.Twelve rats were randomly assigned into two groups and given intragastric administration(9.9 g/kg),after which blood collection was made at different time points,UPLC-MS/MS method was adopted in the determination of plasma concentrations,and main pharmacokinetic parameters were calculated.RESULTS Prolonged Tmax(P＜0.05,P＜0.01)of various active constituents,prolonged T1/2,MRT0-t(P＜0.05,P＜0.01),increased Cmax,AUC(P＜0.05,P＜0.01)and decreased Vd/F,CL/F(P＜0.05,P＜0.01)of paeoniflorin,increased Cmax,AUC(P＜0.05,P＜0.01)and decreased CL/F(P＜0.05)of albiflorin,prolonged MRT(P＜0.05),increased AUC(P＜0.05)and decreased CL/F(P＜0.01)of liquiritin,prolonged MRT(P＜0.05,P＜0.01)and decreased Vd/F(P＜0.05)of isoliquiritin,no obviously changed pharmacokinetic parameters(except for Tmax)of liquiritigenin(P＞0.05),and prolonged T1/2,MRT0-∞(P＜0.05,P＜0.01),increased Cmax,AUC(P＜0.05,P＜0.01)and decreased CL/F(P＜0.01)of glycyrrhizic acid were observable in the model group as compared with those in the normal group.CONCLUSION Gastric ulcer exhibits certain influences on the velocities and degrees of in vivo absorption and metabolism of active constituents from Shaoyao Gancao Decoction.
