Simultaneous content determination of twelve nucleosides in Cervi Cornu slices by LC-MS/MS
10.3969/j.issn.1001-1528.2024.11.002
- VernacularTitle:LC-MS/MS法同时测定鹿角片中12种核苷的含量
- Author:
Xiao-Li SHI
1
;
Bing-Qian FANG
;
Wei LIU
;
Jie SHEN
;
Jin LIU
;
Li LIU
Author Information
1. 上海中医药大学附属曙光医院药学部,上海 201203
- Keywords:
Cervi Cornu slices;
nucleosides;
content determination;
LC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(11):3552-3557
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an LC-MS/MS method for the simultaneous content determination of guanine,adenosine,2′-deoxyuridine,uridine,uracil,thymine,cytidine,xanthine,hypoxanthine,guanosine,inosine and thymidine in Cervi Cornu slices.METHODS The analysis was performed on a 30℃thermostatic Waters X select HSS T3 column(2.1 mm×100 mm,5 μm),with the mobile phase comprising of methanol and 0.1%formic acid flowing at 200 μL/min in a gradient elution manner,and electron spray ionization source was adopted in positive ion scanning with multiple reaction monitoring mode.RESULTS Twelve nucleosides showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 92.46%-104.16%with the RSDs of 2.92%-6.14%.CONCLUSION This stable and feasible method can be used for the quality control of Cervi Cornu slices.
