Protective effect of Naru-3 on collagen-induced arthritis in rats
10.3969/j.issn.1001-1528.2024.06.011
- VernacularTitle:那如-3对胶原诱导性关节炎大鼠的保护作用
- Author:
Hai-Yue ZHAO
1
;
Xiao-Shan ZHANG
;
Sha-Sha DUAN
;
Yi-Lu SHI
;
Min-Jie ZHANG
;
Shu-Rong YUN
;
Ya-Xi WANG
Author Information
1. 内蒙古医科大学附属医院超声科,内蒙古呼和浩特 010050
- Keywords:
Naru-3;
Mongolian medicine;
rheumatoid arthritis;
contrast-enhanced ultrasound;
micro-CT;
X-ray
- From:
Chinese Traditional Patent Medicine
2024;46(6):1842-1849
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the protective effect of Mongolian medicine Naru-3 on rat rheumatoid arthritis(RA)using imaging method.METHODS With the rats divided into the normal group,the model group,the positive medicine group,and the low,medium and high dose Naru-3 groups(0.1,0.2 and 0.4 g/kg),the rat model of collagen-induced arthritis(CIA)was established by immune induction method.After 4 weeks of corresponding drug administration,the rats had their changes of arthritis index(AI)level and body weight observed;their serum levels of VEGF,TNF-α and IL-1 detected by ELISA;their synovial hyperplasia and neovascularization evaluated by high-frequency ultrasound and contrast-enhanced ultrasound(CEUS);their bone destruction of ankle joint evaluated by X-ray and high-resolution micro-CT;and their synovial membrane and expressions of CD31,VEGF,TNF-α and IL-1 β observed by HE and immunohistochemistry.RESULTS Compared with the model group,the Naru-3 groups displayed increased rat weight(P＜0.05);no significantly changed AI score(P＞0.05);and overally decreased levels of serum VEGF,TNF-α,synovial membrane thickness,blood flow signal by power Doppler imaging(PDI)and contrast intensity revealed,X-ray score,and CD31 expression(P＜0.05),in addition to the decreased level of IL-1 and HE score in high-dose group(P＜0.05).CONCLUSION Naru-3 is protective to the joint tissue in rat model of RA through alleviating synovitis,bone erosion and delaying the progress of the disease by inhibiting synovial neovascularization and inflammatory cytokines.
