Effects of hesperidin on gentamicin-induced acute kidney injury,inflammation and apoptosis in mice
10.3969/j.issn.1001-1528.2024.06.010
- VernacularTitle:橙皮苷对庆大霉素致小鼠急性肾损伤及炎症和凋亡的影响
- Author:
Zu-Qing XU
1
;
Shi-Yang WANG
;
Xiao WANG
;
Hua JIN
Author Information
1. 深圳市龙华区中心医院急诊科,广东深圳 518000
- Keywords:
hesperidin;
acute kidney injury;
inflammation;
apoptosis;
PI3K/Akt signaling pathway
- From:
Chinese Traditional Patent Medicine
2024;46(6):1835-1841
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the ameliorative effects of hesperidin on gentamicin-induced acute kidney injury(AKI)in mice,and to explore the correlation between the effects and inflammation and apoptosis.METHODS The mice were randomly divided into the normal group,the model group,the verapamil group(21.43 mg/kg)and the low and high dose hesperidin groups(100,200 mg/kg).With the AKI model established by intraperitoneal injection of gentamicin,the mice of each group underwent 14 days corresponding drug administration.After the intervention,the mice had their levels of 24-hour urine protein,renal index and renal function indexes detected;their levels of IL-10,IL-6 and TNF-α in serum and kidney tissue detected by ELISA;their renal pathological damage detected by HE staining;their renal apoptosis detected by TUNEL staining;their renal mRNA expressions of caspase-3,Bcl-2 and Bax detected by RT-qPCR;and their renal expressions of cleved caspase-3 and PI3K/Akt signaling pathway related proteins detected by Western blot.RESULTS Compared with the model group,the hesperidin groups displayed decreased levels of 24-hour urinary protein and renal index(P＜0.05);improved renal pathological damage in varying degrees;decreased levels of serum BUN,Scr and CysC(P＜0.05);increased IL-10 levels of serum and renal tissue(P＜0.05);decreased levels of IL-6 and TNF-α(P＜0.05);decreased renal apoptosis rate,caspase-3,Bax mRNA expressions and cleaved caspase-3 protein expression(P＜0.05);and increased expressions of Bcl-2 mRNA and PI3K/Akt proteins(P＜0.05).CONCLUSION Hesperidin can ameliorate gentamicin-induced AKI in mice,which is related to its anti-inflammatory efficacy and anti-apoptotic activity via PI3K/Akt signaling pathway.
