Establishment of HPLC fingerprints and content determination of seven constituents for Tanacetum tatsienense
10.3969/j.issn.1001-1528.2024.06.004
- VernacularTitle:川西小黄菊HPLC指纹图谱建立及7种成分含量测定
- Author:
Rui LI
1
;
Wen-Li CHEN
;
Dian-Dian KANG
;
Jie-Yu SUN
;
Ze-Yuan SUN
;
Rui GU
;
Gui-Hua JIANG
Author Information
1. 成都中医药大学药学院,西南特色中药资源国家重点实验室/中药材标准化重点实验室,四川成都 611137
- Keywords:
Tanacetum tatsienense(Bureau & Franchet)K.Bremer & Humphries;
HPLC fingerprints;
chemcial constituents;
content determination;
principal component analysis;
partial least squares discriminant analysis;
cluster analysis
- From:
Chinese Traditional Patent Medicine
2024;46(6):1794-1799
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the HPLC fingerprints for Tanacetum tatsienense(Bureau & Franchet)K.Bremer & Humphries and to determine the contents of chlorogenic acid,quercetin-3-O-glucoside,luteolin-7-O-glucoside,luteolin-7-O-glucuronide,apigenin-7-O-glucuronide,luteolin and apigenin.METHODS The analysis was performed on a 35 ℃ thermostatic Agilent ZORBAX Extend C18 column(4.6 mm×250 mm,0.5 μm),with the mobile phase comprising of 0.5％phosphoric acid-acetonitrile flowing at 1.0 mL/min,and the detection wavelength was set at 350 nm.Subsequently,principal component analysis,partial least squares discriminant analysis and cluster analysis were carried out.RESULTS There were twelve common peaks in the fingerprints for fourteen batches of samples with the similarities of 0.761-0.975.Seven constituents showed good linear relationships within their own ranges(R2≥0.999 1),whose average recoveries were 84.00％-105.11％with the RSDs of 1.28％-2.86％.Various batches of samples were clustered into three types,and seven differential constituents were observable,containing peaks 4(luteolin-7-O-glucoside),12(apigenin),9(apigenin-7-O-glucuronide),8,11(luteolin),5(luteolin-7-O-glucuronide)and 2.CONCLUSION This precise,stable,specific and reproducible method can be used for the quality control of T.tatsienense.
