Simultaneous content determination of eleven constituents and their inhibitory effects on pancreatic lipase for Sanzi Yangqin Decoction
10.3969/j.issn.1001-1528.2024.06.003
- VernacularTitle:三子养亲汤中11种成分含量同时测定及其胰脂肪酶抑制作用研究
- Author:
Hao ZHANG
1
;
Li-Li GONG
;
Jing-Yi WANG
;
Hai-Chao LI
;
Dong-Mei QI
Author Information
1. 山东中医药大学中医药创新研究院,山东济南 250355
- Keywords:
Sanzi Yangqin Decoction;
chemical constituents;
content determination;
pancreatic lipase;
high-performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-QQQ-MS/MS);
molecular docking;
4-nitrophenyl butyrate method
- From:
Chinese Traditional Patent Medicine
2024;46(6):1788-1793
- CountryChina
- Language:Chinese
-
Abstract:
AIM To simultaneously determine the contents of 3,6'-disinapoyl sucrose,sibiricose A5,sibiricose A6,ferulic acid,sinapic acid,caffeic acid,luteolin,progoitrin,apigenin,isorhamnetin and 3,4-dihydroxybenzaldehyde in Sanzi Yangqin Decoction,and to investigate their inhibitory effects on pancreatic lipase.METHODS The high-performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-QQQ-MS/MS)analysis was performed on a 25 ℃ thermostatic Halo-C18column(2.1 mmx100 mm,2.7 μm),with the mobile phase comprising of water(containing 0.05％formic acid)-acetonitrile(containing 0.05％formic acid)flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.The simulation was performed by molecular docking,after which 4-nitrophenyl butyrate method was adopted in the evaluation of inhibitory effects.RESULTS Eleven constituents showed good linear relationships within their own ranges(R2＞0.992 0),whose average recoveries were 85.79％-117.52％with the RSDs of 0.78％-3.73％.3,6'-Disinapoyl sucrose,caffeic acid,sinapic acid and 3,4-dihydroxybenzaldehyde demonstrated good affinities with pancreatic lipase,the IC50 values of Sanzi Yangqin Decoction,caffeic acid,sinapic acid and 3,4-dihydroxybenzaldehyde were 41.16 mg/mL,4.006 mmol/L,6.798 mmol/L and 10.48 mmol/L,respectively.CONCLUSION This stable and reliabe method can provide theoretical basis for the quality control of Sanzi Yangqin Decoction with inhibitory effects on pancreatic lipase.
