Inhibitory effects of tectorigenin on the differentiation of preadipocytes
10.3969/j.issn.1001-1528.2024.05.012
- VernacularTitle:鸢尾黄素对前脂肪细胞分化的抑制作用
- Author:
Ting PAN
1
;
Jie GAO
;
Hui-Hui ZHANG
;
Xing-Hua CAI
;
Tao LEI
;
Jun LU
Author Information
1. 上海中医药大学附属普陀医院内分泌科,上海 200062;成都中医药大学医学与生命科学学院,四川 成都 610000
- Keywords:
tectorigenin;
adipogenesis;
3T3-L1 cells;
lipogenic differentiation
- From:
Chinese Traditional Patent Medicine
2024;46(5):1492-1498
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of tectorigenin on preadipocyte differentiation and the possible mechanism.METHODS CCK8 method was used to detect the effects of tectorigenin on 3T3-L1 cell viability.After 2 days contact inhibition(Day 0 of differentiation),the cells were exposed to inducer and tectorigenin of different concentrations(0,10,20,40,60 μmol/L),in contrast to those of the control group with no inducer use.On the 9th day of differentiation,the cells had their lipid droplets observed by oil red O staining;their levels of TG and NEFA detected by biochemical kit;their protein expressions of PPARγ,C/EBPα,perilipin-1,ADFP,AMPKα and p-AMPKα detected by Western blot;and their mRNA expressions of Adiponectin,FABP4,FAS and Acly detected by RT-qPCR.RESULTS Tectorigenin concentration of 60 μmol/L or lower levels left no impact upon the cell viability(P＞0.05).Compared with the model group with induced differentiation,the groups intervened with tectorigenin administration displayed decreased formation of lipid droplets;lower levels of TG and NEFA(P＜0.01);decreased protein expressions of C/EBPα,PPAR γ,perilipin-1 and ADFP(P＜0.01);increased protein expressions of AMPKα and p-AMPKα(P＜0.01);and decreased mRNA expressions of Adiponectin,FABP4,FAS and Acly(P＜0.01).CONCLUSION Tectorigenin inhibits preadipocyte differentiation into mature adipocytes,which may be related to its efficacy in the regulation of C/EBPα,PPARγ,AMPKα and p-AMPKα.
