Mechanism of icariin inhibiting the proliferation of human prostate cancer PC-3 cells:An exploration based on cell metabolomics
10.13263/j.cnki.nja.2024.11.001
- VernacularTitle:基于细胞代谢组学技术探讨淫羊藿苷抑制人前列腺癌PC-3细胞增殖的机制
- Author:
Tao WANG
1
;
Wei WANG
;
Wen-Jun XIONG
;
Zi-Jing ZHANG
;
Fei WANG
;
Yao-Hui PENG
;
Yan CHEN
;
Hai-Ping ZENG
;
Li-Jie LUO
Author Information
1. 广州中医药大学第一附属医院,广东 广州 510405;广东省中医临床研究院,广东 广州 510405
- Keywords:
icariin;
PC-3 cell;
glycerophospholipid metabolism;
amino acid metabolism;
cell metabolomics
- From:
National Journal of Andrology
2024;30(11):963-973
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the mechanism of icariin inhibiting the proliferation of human PCa PC-3 cells based on cell metabolomics technology.Methods:We determined the proliferation activity of human PC-3 cells by methyl thiazolyl tetrazolium(MTT)assay,and compared the proliferation of the PC-3 cells among the control,5-fluorouracil and icariin intervention groups.Using the Bligh Dyer method,we extracted endogenous metabolites from the cells,analyzed the metabolic profile by ultra-high pressure liquid chromatography tandem quadrupole time-of-flight mass spectrometry,identified the differential metabolites by principal component anal-ysis and orthogonal partial least-squares discrimination analysis,and enriched the metabolic pathways based on the MetaboAnalyst data-base.Results:Icariin significantly inhibited the proliferation of human PCa PC-3 cells.A total of 89 differential metabolites were i-dentified,mainly including amino acids,phosphatidylcholine,phosphatidylethanolamine,lysophosphatidylcholine,and lysophosphati-dylethanolamine,all with the tendency to return to the normal level after icariin intervention.Icariin significantly downregulated the metabolic levels of the glycerophospholipid metabolites phosphatidylcholine,phosphatidylethanolamine,lysophosphatidylcholine and ly-sophosphatidylethanolamine,and upregulated those of amino acid metabolites tryptophan,leucine,and proline in the PC-3 cells.Conclusion:Icariin inhibits the proliferation of human PCa PC-3 cells,which may be closely related to its regulatory effect on lipid metabolism(glycerophospholipid metabolism)and amino acid metabolism.
