Rubusoside protects against myocardial injury in gestational diabetes mellitus rats via the endoplasmic reticulum stress-ferroptosis pathway
10.3760/cma.j.cn311282-20240312-00094
- VernacularTitle:基于内质网应激-铁死亡途径探究甜茶素对妊娠期糖尿病大鼠心肌损伤的保护作用
- Author:
Yinhua YUAN
1
;
Qi GUAN
;
Shanshan ZHANG
Author Information
1. 驻马店市中心医院妇产科，驻马店 463000
- Keywords:
Gestational diabetes mellitus;
Myocardial injury;
Rubusoside;
Endoplasmic reticulum stress;
Ferroptosis
- From:
Chinese Journal of Endocrinology and Metabolism
2024;40(11):956-965
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore effect of rubusoside(RS) on myocardial injury in gestational diabetes mellitus(GDM) rats and its mechanism.Methods:Pregnant SD rats were divided into control, GDM model, insulin treatment(INS), RS, endoplasmic reticulum stress inhibitor 4-phenylbutyric acid(4-PBA), and RS+ endoplasmic reticulum stress inducer thapsigargin(THA) groups. Glycolipid metabolism, cardiac function, serum LDH, cTnI, and CK-MB levels, myocardial tissue morphology, SOD, GSH, MDA, Fe 2+ content were assessed. ROS levels were assessed using DHE staining, and iron ion deposition was evaluated via Prussian blue staining. The expression of endoplasmic reticulum stress and ferroptosis related proteins in myocardial tissue after intervention was analyzed through Western blot and immunohistochemistry. Results:Compared with control rats, GDM model rats showed increased body weight, FBG, TC, TG, abnormal cardiac function, significant myocardial damage, elevated serum LDH, cTnI, CK-MB, reduced SOD and GSH, increased MDA, Fe 2+, ROS, and iron ions, along with altered protein expression indicating endoplasmic reticulum stress and ferroptosis. RS and 4-PBA mitigated these changes, reducing myocardial injury. THA reversed RS′s protective effects via the endoplasmic reticulum stress-ferroptosis pathway. Conclusion:RS improves glycolipid metabolism and alleviates myocardial injury in GDM rats by inhibiting endoplasmic reticulum stress and reducing ferroptosis.
