Effect of propofol on doxorubicin-induced cardiotoxicity in rats
10.3760/cma.j.cn131073.20231201.00812
- VernacularTitle:丙泊酚对阿霉素致大鼠心肌毒性的影响
- Author:
Chen CHENG
1
;
Limei QIAO
;
Zhonghui WANG
;
Liang XIA
;
Jianyun LIU
;
Xiaoyan WANG
Author Information
1. 昆明医科大学第三附属医院（云南省肿瘤医院）麻醉科，昆明 650118
- Keywords:
Propofol;
Doxorubicin;
Cardiotoxicity
- From:
Chinese Journal of Anesthesiology
2024;44(8):963-966
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effect of propofol on doxorubicin-induced cardiotoxicity in rats.Methods:Thirty SPF healthy male Sprague-Dawley rats, aged 2-3 months, weighing 200-220 g, were divided into 3 groups ( n=10 each) by the random number table method: control group (C group), doxorubicin group (D group) and propofol group (P group). The rat cardiotoxicity model was prepared by intraperitoneal injection of doxorubicin 2.5 mg/kg, and the administered dose was adjusted according to the daily body weight once every other day for a total of 6 times in D and P groups. Propofol 50 mg/kg was intraperitoneally injected after successful preparation of the cardiotoxicity model in P group. Blood samples were taken from the heart of the anesthetized rats at 2 h after propofol injection for determination of the concentrations of serum cardiac troponin I (cTnI) and creatine kinase-MB (CK-MB) by enzyme-linked immunosorbent assay. Then the rats were sacrificed, and the myocardial tissues were obtained for microscopic examination of the pathological changes (with a light microscope) and for determination of the mitochondrial volume fraction, surface-area density, surface-area-to-volume ratio, mean mitochondrial area and the number density (by transmission electron microscopy). Results:Compared with C group, the concentrations of cTnI and CK-MB were significantly increased, and the mitochondrial volume fraction, surface-area density, and the number density were decreased in D and P groups ( P<0.05). Compared with D group, the concentrations of cTnI and CK-MB were significantly decreased, and the mitochondrial volume fraction, surface-area density, and the number density were increased in P group ( P<0.05). There was no significant difference in the surface-area-to-volume ratio and mean mitochondrial area among the 3 groups ( P>0.05). Conclusions:Propofol can attenuate doxorubicin-induced myocardial toxicity in rats.
