Effect and mechanism of CD8+T cell subsets on formation of AS in mice
10.3969/j.issn.1009-0126.2024.09.022
- VernacularTitle:CD8+T细胞亚群对小鼠动脉粥样硬化形成的影响及其机制研究
- Author:
Yin ZHANG
1
;
Wei ZHU
;
Bingjun LU
;
Pan GAO
Author Information
1. 400038 重庆,陆军军医大学第一附属医院老年医学与特勤医学科
- Keywords:
atherosclerosis;
T-lymphocyte subsets;
mice,knockout;
CD8+T cell subsets
- From:
Chinese Journal of Geriatric Heart Brain and Vessel Diseases
2024;26(9):1084-1089
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of CD8+T cell subsets on the formation of atheroscle-rosis(AS)in mice.Methods Lasso regression and random forest were employed to identify key infiltrating immune cells in AS blood vessels.Apolipoprotein E gene(ApoE)/recombination acti-vation gene 1(Rag1)double knockout(ApoE/Rag1-DKO)mice were constructed,and then fed with high-fat chow to establish an AS model.The ApoE knockout mice were divided into control group(Con group)and high-fat diet group(HFD group),with 3 mice in each group.And the ApoE/Rag1-DKO mice were divided into DKO+S group[(high-fat diet+phosphate buffer saline(PBS)],DKO+Tem group(high-fat diet with CD8+Tem cell infusion),and DKO+Temra group(high-fat diet with CD8+Temra cell infusion),with 3 mice in each group.Oil red O staining and HE staining were conducted to observe the formation of AS plaques in the vascular walls in the Con,HFD,DKO+S,DKO+Tem,and DKO+Temra groups respectively.Results The results of Lasso regression and random forest showed that CD8+Tem was the most important CD8+T cell subset in the formation of AS.Oil red O staining and HE staining displayed that compared with the Con group,the AS plaque area were significantly larger in the HFD group(P＜0.05);com-pared with the HFD group,there was no significant increase in the AS plaque area in the DKO+S group(P＞0.05);compared with the DKO+S group,the AS plaque area were notably increased in the DKO+Tem group and the DKO+Temra group(P＜0.05).Conclusion CD8+T cell sub-sets are closely associated with the formation of AS in mice,and CD84 Tem and CD84 Temra cells significantly induce the formation.
