Effect of long chain non-coding RNA TUG1 on radiosensitivity of cervical cancer cells by regulating autophagy
10.3760/cma.j.cn113030-20230612-00169
- VernacularTitle:长链非编码RNA TUG1对宫颈癌细胞放射敏感性的影响
- Author:
Yaru WANG
1
;
Dongli ZHANG
;
Changping QU
Author Information
1. 河南大学淮河医院妇产科，开封 475000
- Keywords:
Uterine cervical neoplasms;
Long non-coding RNA;
Taurine upregulated gene 1;
Radiosensitivity;
Autophagy;
Apoptosis
- From:
Chinese Journal of Radiation Oncology
2024;33(5):454-460
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of long non-coding RNA (lncRNA) taurine upregulated gene 1 (TUG1) on the radiosensitivity of cervical cancer cells by regulating autophagy.Methods:The radioresistant cervical cancer cell lines HeLa/IR and SiHa/IR were constructed. The radiosensitivity of HeLa/IR and SiHa/IR cells was evaluated by colony formation assay. Real-time reverse transcription PCR (RT-qPCR) was used to detect the expression of lncRNA TUG1 in each group. Western blot was used to detect the expression of autophagy proteins including Beclin1, microtubule-associated protein1 light chain 3 (LC3)Ⅱ/LC3Ⅰ and p62 in each group. NC-siRNA, TUG1-siRNA, TUG1-siRNA combined with rapamycin (an autophagy activator) were transfected into HeLa/IR and SiHa/IR cells, which were named as NC-siRNA group, TUG1-siRNA group and TUG1-siRNA+rapamycin group, respectively. RT-qPCR was used to evaluate the transfection efficiency of lncRNA TUG1. Western blot was used to assess the effect of lncRNA TUG1 silencing on autophagy protein expression. Flow cytometry was employed to evaluate the effect of lncRNA TUG1 silencing on the proliferation and apoptosis of HeLa/IR and SiHa/IR cells, respectively. The differences between two groups were analyzed by t-test, and the comparison among multiple groups was conducted by one-way analysis of variance. Results:Compared with HeLa and SiHa cells, the survival fractions of HeLa/IR and SiHa/IR cells was significantly increased, the expression of lncRNA TUG1 in cells was significantly increased, the expression levels of autophagy proteins Beclin1 and LC3Ⅱ/LC3Ⅰ were significantly increased, and the expression of p62 protein was significantly decreased, and the differences were statistically significant (all P<0. 05). Compared with the NC-siRNA group, the expression of lncRNA TUG1 and cell viability in HeLa/IR and SiHa/IR cells in the TUG1-siRNA group were significantly decreased, the apoptosis rate was significantly increased, the expression levels of Beclin1 and LC3Ⅱ/LC3Ⅰ proteins were significantly decreased, and the expression of p62 protein was significantly increased, and the differences were statistically significant (all P<0. 05). Compared with the TUG1-siRNA group, the expression levels of Beclin1 and LC3Ⅱ/LC3Ⅰ proteins in HeLa/IR cells in the TUG1-siRNA+rapamycin group were significantly increased, the expression of p62 protein was significantly decreased, the cell viability was significantly decreased, and the apoptosis rate was significantly increased, and the differences were statistically significant (all P<0.05). Conclusion:Silencing lncRNA TUG1 can enhance the radiosensitivity of cervical cancer cells by regulating autophagy.
