Exploration on the Effects of Compound Dihuang Granules on Parkinson Disease Rats with Yin Deficiency and Wind Movement Syndrome Based on PI3K/AKT/mTOR Signaling Pathway
10.19879/j.cnki.1005-5304.202311119
- VernacularTitle:基于PI3K/AKT/mTOR信号通路探讨复方地黄颗粒对帕金森病阴虚动风证大鼠的影响
- Author:
Zhijing ZHANG
1
;
Jianqing LIANG
Author Information
1. 甘肃中医药大学基础医学院,甘肃 兰州 730000
- Keywords:
compound Dihuang Granules;
Parkinson disease;
yin deficiency and wind movement syndrome;
autophagy;
PI3K/AKT/mTOR signaling pathway
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2024;31(7):83-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of compound Dihuang Granules on the Parkinson disease(PD)rats with yin deficiency and wind movement syndrome based on PI3K/AKT/mTOR signaling pathway;To explore the mechanism of its mechanism on PD.Methods A rat model of PD with yin deficiency and wind movement syndrome was prepared by injecting 6-OHDA to substantia nigra.The rats were randomly divided into control group,sham-operation group,model group,madopar group,and compound Dihuang Granules low-,medium-,and high-dosage groups,with 10 rats in each group.Each group was treated with corresponding solution by gavage for 28 consecutive days.After treatment,the neurobehavioral changes of rats were detected,HE staining was used to observe the pathological changes in substantia nigra,and RT-qPCR was used to detect the expressions of PI3K,AKT,mTOR,p62 and LC3B mRNA in damaged substantia nigra,immunohistochemistry was used to detect the expressions of p-PI3K,p-AKT and p-mTOR in damaged substantia nigra,Western blot was used to detect the expressions of p-PI3K,PI3K,p-AKT,AKT,p-mTOR,mTOR,p62 and LC3B protein in damaged substantia nigra.Results Compared with the control group and sham-operation group,the model group rats showed an increase in the number of rotations,an extension of pole climbing time,and a decrease in suspension time and number of moving grids(P<0.01),with disordered arrangement of substantia nigra neurons,nuclear pyknosis,and blurred structure,the expressions of PI3K,AKT,mTOR and p62 mRNA in damaged substantia nigra increased,the protein expressions of p-PI3K,p-AKT,p-mTOR and p62 increased,and the expressions of LC3B mRNA and LC3BⅡ/Ⅰ protein decreased(P<0.01).Compared with the model group,the number of rotations and pole climbing time of rats in madopar group and compound Dihuang Granules groups decreased,while the suspension time and number of moving grids increased(P<0.01),with increased number of substantia nigra neurons and improved structural morphology,the expressions of PI3K,AKT,mTOR and p62 mRNA in damaged substantia nigra decreased,the protein expressions of p-PI3K,p-AKT,p-mTOR and p62 decreased,and the expressions of LC3B mRNA and LC3BⅡ/Ⅰ protein increased(P<0.05,P<0.01).The compound Dihuang Granules high-dosage group had the most significant effect(P<0.05,P<0.01),and there was no statistically difference compared with the madopar group(P>0.05).Conclusion Compound Dihuang Granules may enhance damaged substantia nigra autophagy by inhibiting the PI3K/AKT/mTOR signaling pathway,protect dopaminergic neurons,and treat PD.
