Optimization and evaluation of detection method for related substances in bucladesine sodium
10.19778/j.chp.2024.05.005
- VernacularTitle:二丁酰环磷腺苷钠有关物质检测方法优化及评价
- Author:
Bing ZHAO
1
;
Li MA
;
Lingxu YE
;
Qingting YU
;
Qiuyun SHEN
;
Xiangfeng WANG
Author Information
1. 湖南尔康制药股份有限公司,长沙 410331
- Keywords:
bucladesine sodium;
HPLC;
related substances;
cyclophosphaminate sodium;
N6-butyryl 3',5-cyclo-phosphatin sodium;
2'-0 butyryl 3,5-cycloadenosine monophosphate sodium
- From:
Drug Standards of China
2024;25(5):458-464
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish and optimize a detection method of dibutyryl cyclic adenosine phosphate related substances.Methods:On the basis of the JP,the experimental conditions were optimized to obtain the optimal detection conditions.The column was CAPCEILPAK C18;mobile phase was pH 6.0 phosphate buffer solution-meth-anol with gradient elution;detection wavelength was 258 mm.The method validation was carried out.Results:In the system applicability solution,the separation degree of the main peak and adjacent peaks in each destruction solution was greater than 1.5;the solution was stable within 48 h.The content of each impurity showed a good line-ar relationship with the peak area in the range of 50％-150％of the limit concentration,R2＞0.999 4.In the spike experiment,the average recovery rate of N6-butyryl 3',5-cyclophosphatin sodium was 99.4％-106.6％.The average recoveries of 2'-0 butyryl 3,5-cycloadenosine monophosphate sodium were 88.8％-103.6％,and the average recoveries of cyclophosphaminate sodium were 96.9％-102.5％,which met the requirements.Three bat-ches of samples were tested,and the above impurities were detected.Conclusion:This method has high sensitivi-ty,good separation effect,accuracy and durability,and can be used for the detection of bucladesine sodium,provi-ding a basis for its quality control.
