Pterostilbene inhibits the growth of esophageal squamous cell carcinoma by targeting PPARα signaling pathway and inducing ferroptosis
- VernacularTitle:紫檀芪通过靶向PPARα信号通路促进铁死亡抑制食管鳞癌细胞生长
- Author:
Yi YANG
1
;
Wen-Jie SHI
;
Shan LI
;
Yue ZHANG
;
Yuan-Qian MIN
;
Bao-Ping LU
Author Information
- Keywords: pterostilbene; proteomic analysis; esopha-geal squamous cell carcinoma(ESCC); PPARα signa-ling pathway; ferroptosis; bioinformatics analysis
- From: Chinese Pharmacological Bulletin 2024;40(12):2354-2360
- CountryChina
- Language:Chinese
- Abstract: Aim To study the molecular mechanism of pterostilbene(PTS)inhibiting the growth of esophage-al squamous cell carcinoma(ESCC).Methods Soft agar assay was used to detect the effect of PTS on the anchored independent growth of KYSE150.TMT-la-beled quantitative proteomics analysis was used to ana-lyze the influence of PTS on the proteome of KYSE150.Then the differentially expressed proteins(DEPs)enrichment was analyzed by GO and KEGG,and signaling pathway interactions were analyzed by STRING database.The molecular docking model of PTS and PPARα was established by computer.Trans-mission electron microscopy was used to observe the in-fluence of PTS on the morphology change of KYSE150.Western blot analysis the effects of PTS on PPARα sig-naling pathway and ferroptosis related proteins expres-sion.Results PTS inhibited the anchorage-independ-ent growth capability of KYSE150.A total of 249 DEPs were identified by proteomic analysis,including 175 up-regulated proteins and 74 down-regulated pro-teins.The DEPs enrichment analysis showed that PPAR signaling pathway was related to unsaturated fat-ty acid synthesis,pyruvate metabolism and other meta-bolic signaling pathways.PTS caused the reduction of mitochondrial volume and mitochondrial cristae of KYSE150.PTS inhibited the expression of PPARα sig-naling pathway and ferroptosis related proteins.Con-clusion PTS induced the ferroptosis of ESCC by in-hibiting PPARα signaling pathway.
