Mechanism of circ_0103552 regulating lung cancer A549 cell proliferation,migration and invasion by targeting miR-1200
10.12360/CPB202406042
- VernacularTitle:Circ_0103552靶向miR-1200调控肺癌A549细胞增殖、迁移及侵袭
- Author:
Fang ZHOU
1
;
Meng WANG
;
Shi-Zhao CHENG
;
Zheng WANG
;
Si-Pei ZHANG
;
Xi-Ke LU
Author Information
1. 天津大学胸科医院胸外科,天津 300222
- Keywords:
lung cancer;
circ_0103552;
miR-1200;
proliferation;
migration;
invasion
- From:
Chinese Pharmacological Bulletin
2024;40(11):2099-2104
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of circ_0103552 on the proliferation,migration and invasion of lung cancer A549 cells and its possible mechanism.Meth-ods qRT-PCR was used to detect circ_0103552 and miR-1200 expression in lung cancer tissues and adja-cent tissues.A549 cells were grouped into si-NC,si-circ_0103552,miR-NC,miR-1200,si-circ_0103552+anti-miR-NC,si-circ_0103552+anti-miR-1200 groups.Cell proliferation,clone formation,migration and invasion were detected.The targeting relationship between circ_0103552 and miR-1200 was assessed u-sing the dual luciferase reporter experiment.The ex-pression of E-cadherin and N-cadherin protein was de-tected by Western blot.Results Compared with adja-cent tissues,circ_0103552 expression in lung cancer tissue increased(P＜0.05),while miR-1200 expres-sion decreased(P＜0.05).Circ_0103552 knockdown or miR-1200 overexpression reduced cell viability,N-cadherin,cloning,invasion,and promoted E-cadherin protein(P＜0.05).Circ_0103552 could target miR-1200.Down-regulating miR-1200 could reverse the in-hibitory effect of circ_0103552 knockdown on prolifera-tion,migration and invasion,as well as the promotion effect on cell apoptosis(P＜0.05).Conclusion Circ_0103552 could promote lung cancer cell progres-sion by targeting miR-1200.
