Mechanism of quercetin inhibiting neutrophil extracellular trap formation based on LOX-1/NOX2 signaling pathway
- VernacularTitle:基于LOX-1/NOX2信号通路探讨槲皮素抑制中性粒细胞胞外诱捕网形成机制
- Author:
Si LIU
1
;
Gang LUO
;
Jun-Chang WANG
;
Lin-Yao YING
;
Yan WANG
;
Lin XIAO
Author Information
- Keywords: inflammation; lysophosphatidylcholine; neutrophil extracellular traps; quercetin; LOX-1; NOX2
- From: Chinese Pharmacological Bulletin 2024;40(11):2047-2054
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the molecular mecha-nism of lysophosphatidylcholine(LPC)-induced neu-trophil extracellular trap formation and the intervening role of quercetin.Methods An in vitro model of NETs formation was constructed using LPC-induced primary rat neutrophils,and quercetin intervention was performed.The formation of NETs and the expression level of related pathway proteins were detected.Results 100 mg·L-1 LPC induced the formation of NETs in vitro,and NETs formation was associated with the up-regulation of LOX-1/NOX2 pathway protein expres-sion.Quercetin intervention effectively decreased LOX-1 and NOX2 protein expression,then inhibiting NETs formation.Conclusions Quercetin inhibits the forma-tion of NETs by regulating the LOX-1/NOX2 signaling pathway and exerting a protective effect against related inflammatory diseases.
