Effect of Tripterygium wilfordii polyglycosides on renal protection and expression of CB2R/NOX4/NLRP3 in renal tissue of IgA nephropathy rats
- VernacularTitle:雷公藤多苷对IgA肾病大鼠肾脏保护及肾组织CB2R/NOX4/NLRP3表达的影响
- Author:
Chen-Chen CHEN
1
;
Ke SONG
;
Ying DING
;
Wen-Jing ZHAO
;
Shou-Lin ZHANG
;
Chun-Dong SONG
Author Information
- Keywords: IgA nephropathy; Tripterygium wilfordii polyglycosides; CB2R/NOX4/NLRP3; endogenous cannabinoid system; oxidative stress; inflammatory re-action
- From: Chinese Pharmacological Bulletin 2024;40(11):2037-2041
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of Tripterygi-um wilfordii polyglycosides(TWP)on renal protection and the expression of cannabinoid 2 receptor/NADPH oxidase4/NOD like receptor protoin3(CB2R/NOX4/NLRP3)in renal tissue of IgA nephropathy(IgAN)rats.Methods The IgAN rat model was replicated u-sing the"BSA+CCl4+LPS"combined method,and intervention with TWP was administered.A fully auto-mated biochemical analyzer was used to detect 24-hour urine protein quantification(24h-UTP),urine red blood cell count(URBC),serum albumin(ALB),ala-nine aminotransferase(ALT),serum creatinine(Scr),and blood urea nitrogen(BUN).The pathological changes in renal tissue were observed under light mi-croscopy,and IgA deposition in the mesangial area was observed using immunofluorescence.The protein ex-pressions of CB2R,NOX4 and NLRP3 in renal tissue were detected by Western blot.Results Compared with the model group,the pathological damage to the kidneys of rats in the TWP group was significantly re-duced,and the deposition of IgA electron dense materi-al was significantly reduced.The levels of ALB in rats treated with TWP increased,while the levels of 24h-UTP,URBC,ALT,Scr,and BUN all decreased.The expression of CB2R protein in renal tissue of rats trea-ted with TWP increased,while the expression of NOX4 and NLRP3 proteins was reduced.Conclusion TWP can effectively improve renal injury in IgAN rats,and its mechanism may be related to the regulation of the CB2R/NOX4/NLRP3 signaling pathway.
