Mechanism of DanShenyin in heart failure based on network pharmacology and experimental validation
10.12360/CPB202402042
- VernacularTitle:基于网络药理学和实验验证研究丹参饮治疗心衰的作用机制
- Author:
Jing WANG
1
;
Li LI
;
Bing LIU
;
Kun ZHOU
;
Ying-Li YU
Author Information
1. 天津中医药大学药物安全评价中心
- Keywords:
DanShenyin;
heart failure;
network phar-macology;
ultrasound;
mechanism of action;
molecular docking
- From:
Chinese Pharmacological Bulletin
2024;40(9):1773-1780
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the pharmacological effects of Danshenyin(DSY)in treating heart failure using network pharmacology and in vitro experiments.Methods Utilizing the Traditional Chinese Medicine Systems Pharmacology Analysis Platform(TCMSP),UniProt and other databases,active components and targets of DSY were analyzed.Disease-related targets were identified via searches in the Gene Cards and oth-er databases using keyword"heart failure".Subse-quently,the intersection of DSY and heart failure tar-gets was conducted to obtain core targets.The STRING database was employed to construct a protein-protein interaction(PPI)network,and biological function and signal pathway enrichment analysis were performed u-sing the DAVID database.Establish a network of"me-dicinal materials-ingredients-target-disease."Accord-ing to this network,a preliminary docking validation of the active ingredient with the target molecule.A mouse model of chronic heart failure was established through left anterior descending coronary artery ligation,with prediction results confirmed via echocardiographic de-tection and Western blotting.Results Network phar-macological analysis indicated that DSY may regulate the MAPK cascade signaling pathway via pharmacologi-cal components such as danshenyin aldehyde,luteolin,tanshinone A,and miltionone Ⅱ to treat heart failure.Animal experiments demonstrated significant improve-ments in ultrasound indexes like left ventricular cardiac function and outflow tract blood flow in the DSY treat-ment group compared to the model group(P＜0.05).Additionally,myocardial tissue phosphorylation levels of ERK,JNK,and p38 were significantly reduced(P＜0.05).Conclusion DSY protects the myocardi-um,influences the MAPK pathway,and holds promise for treating chronic heart failure.
