Effects of nalbuphine on the activity and angiogenesis of colorectal cancer cells by regulating the HIF-1α/VEGF sig-naling pathway
10.3969/j.issn.1009-9905.2024.10.002
- VernacularTitle:纳布啡调节HIF-1α/VEGF信号通路对结直肠癌细胞活性和血管生成的影响
- Author:
Yuan-Yuan TIAN
1
;
Bei WU
;
Fei WANG
;
Jing YANG
Author Information
1. 保定市第二医院麻醉科(河北 保定 071051)
- Keywords:
Colorectal cancer;
Nabphine;
Hypoxia-inducible factor-1α/vascular endothelial growth factor signaling pathway;
Proliferation;
Migration;
Invasion;
Angiogenesis
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(10):760-765
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of nalbuphine(Nal)on the activity and an-giogenesis of colorectal cancer(CRC)cells by regulating the hypoxia inducible factor-1 α(HIF-1 α)/vascular endothelial growth factor(VEGF)signaling pathway.Methods:CRC cell SW480 was treated with Nal of 25-800 μmol/L to screen for the optimal drug concentration;CRC cells were separated into Control group,low concentration nalbuphine group(Nal-L group,50 μmol/L),me-dium concentration nalbuphine group(Nal-M group,100 μmol/L),high concentration nalbuphine group(Nal-H group,200 μmol/L),and high concentration nalbuphine(200 μmol/L)+HIF-1 α/VEGF signaling pathway activator group(Nal-H+DMOG group,200 μmol/L Nal+2 mM DMOG).The cell plate cloning experiment was applied to detect cell proliferation.The apoptotic ability of cells was evaluated by flow cytometry.The migration ability of cells was tested using scratch assay.The in-vasive ability of cells was measured using the Transwell method.The ability of cells to form blood vessels and the expression of HIF-1αA/EGF signaling pathway proteins were analyzed using Western blot.Results:Nal can inhibit cell proliferation in a concentration dependent manner.Compared with the Control group,the number of clone formation,scratch healing rate,number of invasive cells,number of vascular structure formation,HIF-1α,and VEGF protein expression in the Nal-L group,Nal-M group,and Nal-H group were greatly reduced(P＜0.05),the apoptosis rate of cells was obviously increased(P＜0.05).Compared with the Nal-H group,the number of clone formation,scratch healing rate,number of invasive cells,number of vascular structure formation,HIF-1α,and VEGF protein expression in the Nal-H+DMOG group were obviously increased(P＜0.05),the apoptosis rate of cells was greatly decreased(P＜0.05).Conclusion:The inhibitory ef-fects of Nal on proliferation,migration,invasion,and vascular structure formation of CRC cells may be achieved by inhibiting the HIF-1αNEGF signaling pathway.
