Expression of miR-1247-5p in breast cancer tissue and bio-informatics prediction of its target gene
10.3969/j.issn.1009-9905.2024.06.006
- VernacularTitle:乳腺癌组织miR-1247-5p表达及其靶基因生物信息学预测研究
- Author:
Fang CHEN
1
;
Ge-Yang WANG
;
Fang-Jing MA
Author Information
1. 新疆医科大学第一附属医院 乳腺外科(新疆 乌鲁木齐 830000)
- Keywords:
Breast neoplasms;
MicrokNA-1247-5-p;
Target gene;
Bioinformatics
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(6):453-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of miR-1247-5p(miR-1247-5p)in breast cancer tissues,and to analyze its predicted target genes by bioinformatics.Methods:The cancerous tissues and adjacent tissues were collected from 104 patients with breast cancer who received surgical treatment in our unit from June 2019 to December 2020.The relative expression of miR-1247-5p was detected by RT-PCR.The expression of miR-1247-5p in cancer tissues of patients with different clinicopathological characteristics was compared.The target genes of miR-1247-5p were predicted.The DAVID database was used for function and pathway enrichment of miR-1247-5p target genes.The String database was utilized to analyze the interactions of miR-1247-5p target genes.Results:The expression level of miR-1247-5p in breast cancer tissue was lower than that in adjacent tissue(P＜0.05).There were 38 potential target genes of miR-1247-5p,and the functions of its target genes are mainly concentrated in transcriptional regulation of RNA polymerase Ⅱ promoter,gene expression/transcriptional regulation,plasma membrane portion,synaptosome,protein binding,and poly(A)RNA binding,etc.The involved pathways were mainly enriched in cancer pathway,interaction between cytokines and cytokine receptors,and Phosphatidylinositol 3 kinase(PI3K)-protein kinase B(Akt)signal pathway,etc.Phosphatase and tensin homology(PTEN),epithelial growth factor receptor(EGFR),pseudouridine synthase 1(PUS1)encoding protein were found in the key node positions of miR-1247-5p gene target gene protein-protein interaction networks(PPI).Conclusion:miR-1247-5p is down-regulated in breast can-cer tissues,and its target genes are enriched in multiple biological processes and tumor-related signal transduction pathways.miR-1247-5p may participate in the occurrence and development of breast cancer by regulating the expression of its target genes.
