Effect of vitamin K2 on chemoresistance of cholangiocarci-noma cells by regulating YAP/TAZ signal pathway
10.3969/j.issn.1009-9905.2024.04.003
- VernacularTitle:维生素K2调节YAP/TAZ信号通路对胆管癌细胞化疗耐药性的影响
- Author:
Yu-Wei YAN
1
;
Wen SHI
;
Dan HU
Author Information
1. 南通市第一人民医院临床营养科(江苏 南通 226000)
- Keywords:
Cholangiocarcinoma;
Vitamin K2;
Yes-associated protein/transcriptional co-activa-torwith PDZ-binding motif signal pathway;
Chemotherapy resistance
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(4):264-269
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of vitamin K2(VK2)on the chemotherapy resis-tance of cholangiocarcinoma(CCA)cells by regulating the Yes-associated protein(YAP)/transcriptional co-activator with PDZ-binding motif(TAZ)signal pathway.Methods:The QBC939 cell was set as the Control group(blank medium treatment),QBC939/ADM cells were randomly divided into 5-fluo-rouracil(5-FU)resistant group(5-FU group),low concentration VK2 group,medium concentration VK2 group,high concentration VK2 group,and high concentration VK2+YAP activator JASP group.CCK-8 was used to detect the cell proliferation.Scratch test was used to detect cell migration.Transwell assay was used to detect the invasiveness of cells.Apoptosis was detected by flow cy-tometry.Western Blot was used to detect the expression of apoptosis related proteins Bax,Bcl-2,Caspase3 and YAP/TAZ signal pathway related proteins.Results:Compared with the control group,the cell OD 450 value(48 h,72 h),cell migration rate,number of cell invasion,and the ex-pression of YAP,TAZ,Bcl-2 proteins in 5-FU group increased obviously(P＜0.05),the apoptosis rate,and the expression of Bax and Caspase-3 proteins decreased obviously(P＜0.05).Compared with 5-FU group,the changes of corresponding indicators of cells in VK2-M group and VK2-H group were contrary to the above(P＜0.05).JASP attenuated the reversal effect of VK2 on CCA chemoresistance.Conclusion:VK2 may reverse the resistance of CCA cells to chemotherapy drugs by inhibiting YAP/TAZ signal pathway.
