Effects of anthoxanthin on the growth,proliferation and in-vasion of human gastric cancer cell line SGC7901
10.3969/j.issn.1009-9905.2024.04.002
- VernacularTitle:芒柄花黄素对人胃癌细胞系SGC7901生长、增殖及侵袭影响
- Author:
Hao-Yu ZHANG
1
;
Xin KANG
;
Bin LI
;
Tian-Sheng YIN
;
Ya-Jun ZHANG
Author Information
1. 成都市双流区第一人民医院 普外科(四川 成都 616200)
- Keywords:
Gastric cancer;
SGC7901;
Formononetin;
Apoptosis;
Wnt/β-Catenin channel
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(4):259-263
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of anthoxanthin on the proliferation and apopto-sis of human gastric cancer cell line SGC7901 and its possible mechanism.Methods:SGC7901 was cultured in the medium containing different concentrations(0,20,40,80 μ mol/L)of antho-cyanin.Hoechst staining,MTT colorimetry and flow cytometry were used to verify the effect of Form on the inhibition rate,cell cycle,nuclear morphology and apoptosis.Western Blotting was used to test the level of β-Catenin、p-β-Catenin,CyclinD1,CDK4,p-AKT,AKT,BCL-2,BAX,caspase3 and other proteins.The mRNA expression of BCL-2,CDK4,CDK6,AKT,CyclinD1,BAX,Caspase3 was detected by RT-PCR.Results:MTT results showed that the inhibitory effect of Form on SGC7901 was concentration dependent and time dependent.Hoechst staining showed that the cells in the experimental group(20,40,80 μ mol/L),the phenomenon of pyknosis and dissolution of the nucleus,even granular aggregation after rupture.Flow cytometry showed that the apoptosis rate of the experimental group(20 μ mol/L,40 μ mol/L,80 μ mol/L)was 17.0％,21.5％,32.5％respectively after treated with different concentrations of Form for 48 hours,which was positively correlated with the drug concentration.RT-PCR showed that caspase3 and BAX mRNA expression increased with the increase of Form intervention concentration(P＜0.01);the mRNA expression of BCL-2,CDK4,CDK6,AKT,CyclinD1 decreased(P＜0.01).WB results showed that β-Catenin、p-β-Catenin,Cy-clinD1,CDK4 and p-AKT,AKT,BCL-2 decreased with the increase of Form intervention concentra-tion(P＜0.01);The relative expression of caspase3,BAX and other proteins increased(P＜0.01).Con-clusion:Form can promote apoptosis of SGC7901,and the possible mechanism is to inhibit Wnt/β-Catenin signal;it also mediates the activation of Caspase family.
