Expression levels and clinical significance of miR-34a in the plasma of patients with diabetic kidney disease
10.3969/j.issn.1006-6187.2024.05.009
- VernacularTitle:糖尿病肾脏疾病患者血浆miR-34a表达水平及临床意义
- Author:
Wenhua HU
1
;
Mengmeng WU
Author Information
1. 712100 咸阳,杨凌示范区医院内分泌科
- Keywords:
Diabetic kidney disease;
miR-34a;
Receiver operating characteristic curve
- From:
Chinese Journal of Diabetes
2024;32(5):361-363
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression level of plasma miR-34a in patients with diabetic kidney disease(DKD)and its clinical significance.Methods 189 type 2 diabetes mellitus(T2DM)patients treated in our hospital from June 2019 to June 2020 were selected and divided into normal proteinuria group(Nor,UACR<30 mg/g,n=88),microalbuminuria group(Mic,30≤UACR<300 mg/g,n=60)and macroproteinuria group(Mac,UACR≥300 mg/g,n=41)based on the levels of UACR.,During the same period,healthy individuals who underwent physical examination were selected as the normal control group(NC,n=90).Neutrophil gelatinase-associated lipocalin(NGAL),kidney injury molecule 1(KIM-1)were measured by ELISA.The expression of miR-34a in plasma was measured by reverse transcription-poly-merase chain reaction,The diagnostic value of DKD was analyzed by receiver operating characteristic(ROC)curve.Results The levels of FPG,HbA1c,Scr,BUN,NGAL,UACR,KIM-1 and miR-34a in NC,Nor,Mic and Mac groups increased sequentially(P<0.05).Pearson correlation analysis showed that miR-34a was positively correlated with FPG,HbA1c,Scr,BUN,NGAL,UACR and KIM-1(P<0.01).The area under ROC curve was 0.824,with the sensitivity 75.1%and specificity 86.7%,respectively.Conclusion Plasma miR-34a has certain diagnostic value for DKD.
