Analysis of RhC Antigen Weak Expression Combined with Mimicking Autoanti-Ce and Homologous Anti-Jkb Causing Mismatch
10.19746/j.cnki.issn1009-2137.2024.05.036
- VernacularTitle:RhC抗原弱表达伴类自身抗-Ce及同种抗-Jkb致配血不合分析
- Author:
Hong-Mei YANG
1
;
Xi YU
;
Xin ZOU
;
Si-Fei MA
;
Jin CHEN
;
Jian-Wei ZHANG
Author Information
1. 常州市中心血站,江苏常州 213000;常州市临床输血重点专科实验室,江苏常州 213000
- Keywords:
genetic recombination;
antibody screening;
mimicking anti-Ce;
anti-Jkb
- From:
Journal of Experimental Hematology
2024;32(5):1539-1544
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the reasons for the weak expression of RHCE gene in a patient whose mimicking anti-Ce combined with anti-Jkb caused cross-matching non-combination.Methods:ABO,Rh,and Kidd blood group antigens were identified by test tube method and capillary centrifugation.Antibody screening and antibody specificity identification were performed using saline,polybrene and antiglobulin in tri-media association with multispectral cells.RHCE gene sequencing and haploid analysis were performed by multiplex PCR technique and RHCE protein modeling was performed using Swiss-Model.Results:The serum of the patient contained anti-Ce mimicking autoantibodies along with anti-Jkb antibodies.c.48G＞C,c.150C＞T,c.178C＞A,c.201A＞G,c.203A＞G,and c.307C＞T mutations were detected in the RHCE triple-molecule sequencing.A 109 bp insertion sequence was found in intron 2,with fragment loss from intron 5-8.The Rh-group genotype was DCe/DCe,and phenotype was CCDee.Conclusion:Genotyping techniques can assist in deducing the molecular mechanisms of some weakly expressed RhC,c,E,and e in patients'sera to aid in the identification of difficult antibodies and thus ensure the safety of patients'blood transfusion.
