The Effects of RNF213 on the Proliferation and Apoptosis of Acute Myeloid Leukemia THP-1 Cells
10.19746/j.cnki.issn1009-2137.2024.05.009
- VernacularTitle:RNF213基因对急性髓系白血病THP-1细胞增殖及凋亡的影响
- Author:
Xiao-Qi SHI
1
;
Ping-Ping ZHANG
;
Ya-Ning GUAN
;
Zuo-Chen DU
;
Yan CHEN
;
Pei HUANG
;
Zhi-Xu HE
Author Information
1. 遵义医科大学附属医院小儿内科,贵州省儿童医院小儿内科,遵义医科大学组织损伤修复与再生医学省部共建协同创新中心,贵州遵义 563000
- Keywords:
RNF213 gene;
acute myeloid leukemia;
prognosis;
proliferation;
apoptosis
- From:
Journal of Experimental Hematology
2024;32(5):1365-1371
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To discover the relationship between the RNF213 gene and acute myeloid leukemia(AML),and explore the effect of RNF213 on the proliferation and apoptosis of THP-1 cells.Methods:Analyze the expression of RNF213 gene in AML and its relationship with prognosis through the GEPIA database.Collecting 30 AML patients and non-tumor hematological patients who went to the Affiliated Hospital of Zunyi Medical University from January 2017 to January 2022.RT-qPCR and Western blot were used to detect the expression levels of RNF213 mRNA and protein.Perform survival of patients was analysed by Kaplan-Meier.Meanwhile,the expression levels of RNF213 mRNA and protein were detected in AML cell lines(THP-1,OCI-AML2).CRISPR-Cas9 was used to knockdown the RNF213 gene in THP-1 cells;flow cytometry was used to detect apoptosis rate of cell.CCK-8 and colony formation assay were used to detect cell proliferation.Western blot was used to detect the expression level of Cleaved-Caspase 3 protein.Results:Compared with the control group,the expression level of RNF213 in AML patients was significantly increased,and patients with high expression of RNF213 have a worse prgnosis.Higher expression level of RNF213 protein in THP-1 cells.After knocking down the RNF213 gene of THP-1 cells,cell proliferation was significantly reduced,and the apoptosis rate and expression of apoptosis related protein Cleared-Caspase3 were significantly increased.Conclusion:AML patients have high expression of RNF213,and the prognosis of high expression patients is poor.The RNF213 gene affects AML cell proliferation and apoptosis,and may be a prognostic marker and potential therapeutic target for AML.
