Relative Quantification for Obtaining the IgG Blood Group Antibodies in Plasma by the Absorption and Elution Test
10.19746/j.cnki.issn1009-2137.2024.04.046
- VernacularTitle:吸收放散法相对定量获取血浆IgG血型抗体的研究
- Author:
Xi QI
1
;
Lin-Bao GAO
;
Sheng-Xia CHEN
Author Information
1. 江苏大学医学院检验系,江苏镇江 212013;泰州市人民医院输血科,江苏泰州 225300
- Keywords:
absorption and elution test;
IgG blood group antibody;
ABO blood group incompatibility in maternity and infant;
hemolytic disease of the fetus and newborn
- From:
Journal of Experimental Hematology
2024;32(4):1278-1283
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the absorption and elution test for relatively quantitive obtaining anti-A and anti-B blood group IgG antibodies in the plasma of O-type RhD-positive pregnant women.Methods:95 cases of the O-type RhD-positive pregnant women plasma samples were randomly selected for obtaining the IgG antibodies of anti-A and anti-B blood group,with absorption test under 37 ℃ and elution test under 56 ℃,and the IgG anti-A and anti-B antibody titers of plasma and elution were determined by the microcolumn gel anti-human globulin test.The differences and correlation between the titers of IgG antibodies in the eluent and plasma were compared and analyzed.Results:After a logarithmic transformation(Log2),there was no statistically difference between IgG antibody anti-A difference value and anti-B difference value in the eluent and plasma(P＞0.05).The titer of IgG antibody in the eluent was positively correlated with the titer of IgG antibody in the plasma(r=0.914).The linear equation for IgG antibody titers fitted by a scatter plot between the eluent and plasma was Y=-3.55+0.96X.Conclusion:The absorption and elution test can be used to obtain the anti-A and anti-B IgG antibodies in the plasma of O-type RhD-positive pregnant women,whose plasma origin IgG titer is greater than 8.Meanwhile,the acquisition of anti-A antibodies was as effective as anti-B antibodies at the same time,and the antibodies obtained are positive proportional to their respective concentrations in the plasma.
