Expression and Significance of PD-1/PD-L1 in MDS Blast Cells,T Lymphocyte Cell Subsets and Treg Cells
10.19746/j.cnki.issn1009-2137.2024.04.027
- VernacularTitle:PD-1/PD-L1在MDS原始细胞、T细胞亚群和Treg细胞中的表达及意义
- Author:
Yue-Fei WANG
1
;
Bao-Guo CHEN
;
Rui ZHENG
;
Yi-Ni YU
Author Information
1. 浙江大学附属二院临海分院(临海市第一人民医院)儿内科
- Keywords:
myelodysplastic syndromes;
PD-1/PD-L1;
T lymphocyte cell subsets;
Treg cells
- From:
Journal of Experimental Hematology
2024;32(4):1152-1159
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression and significance of PD-1/PD-L1 in MDS blast cells,T lymphocyte cell subsets and Treg cells.Methods:Eighty-eight MDS patients and 19 AML patients were collectd as the study subjects,and Iron deficiency anemia and healthy bone marrow donors were used as control group.The expression of PD-1/PD-L1 in MDS/AML blast cells,T lymphocyte cell subsets and Treg cells was detected by flow cytometry,and the expression level of Th1/Th2/Th17-related cytokines in peripheral serum was detected.Results:The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells in low risk MDS group were lower than that in control group,medium and high risk MDS group and AML group(all P＜0.01),and Th1/Th17 type cytokines were dominant.The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells of intermediate and high risk MDS group and AML group were higher than that of control group and low risk MDS group(all P＜0.01),and Th2 type and Treg type(IL-10、TGF-β)cytokines were dominant.After treatment,the differences of PD-1/PD-L1 expression were not statisticatly significant in blast cells,T lymphocyte cell subsets and Treg cells between the MDS remission group and the control group(all P＞0.05).The expression of PD-1/PD-L1 in blast cells,T lymphocyte cell subsets and Treg cells in MDS non-remission group were significantly higher than that in remission group and control group(all P＜0.01).Conclusion:The high expression of PD-1/PD-L1,dominance of Treg(IL-10、TGF-β)and Th2-related cytokines and inhibition of effector T lymphocyte cells in patients with MDS is conducive to tumor cell proliferation and immune escape,which may promote the progression of MDS disease.
