Expression and Function of circ_0073585 in Acute Myeloid Leukemia
10.19746/j.cnki.issn1009-2137.2024.04.007
- VernacularTitle:circ_0073585在急性髓系白血病中的表达及功能研究
- Author:
Yong-Qing ZHOU
1
;
Xiao-Yu SU
;
Qian ZHAO
;
Zhao-Qun DENG
Author Information
1. 江苏大学附属人民医院中心实验室,镇江市精确诊断与治疗重点实验室,江苏镇江 212002
- Keywords:
circular RNA;
circ_0073585;
acute myeloid leukemia
- From:
Journal of Experimental Hematology
2024;32(4):1018-1025
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression level,clinical significance and function of circular RNAs(circRNAs)circ_0073585 in the bone marrow of patients with acute myeloid leukemia(AML).Methods:The expression levels of circ_0073585 in bone marrow samples of 106 newly diagnosed AML patients and 38 controls were detected by real-time quantitative PCR(RQ-PCR).The differences were compared between the two groups and their clinical significance was analyzed.The diagnostic value of circ_0073585 expression for AML was evaluated by receiver operating characteristic curve(ROC).THP-1 cells with lentivirus overexpressing circ_0073585 vector and empty vector were divided into two groups:circ_0073585-THP-1 and NC-THP-1 group.CCK-8 assay and flow cytometry were used to study the effects of circ_0073585 on THP-1 cell proliferation,survival,apoptosis and drug sensitivity.Results:Compared with the controls,the expression level of circ_0073585 in the bone marrow of AML patients was significantly reduced(P＜0.001).There was a statistically significant difference between the high and low expression groups of circ_0073585 in the white blood cell count,platelet count(P＜0.01)and chromosome risk(P＜0.05).Compared with NC-THP-1 cells,the proliferation and viability of circ_0073585-THP-l cells were weakened(P＜0.01),the apoptosis rate was increased(P＜0.01),and the sensitivity to homoharringtonine(P＜0.05)and daunorubicin hydrochloride(P＜0.001)was increased.Conclusion:The expression level of circ_0073585 is decreased in AML patients.Overexpression of circ_0073585 can inhibit the proliferation and viability of leukemic cells,promote apoptosis,and increase sensitivity of leukemia cells to chemotherapy drugs.
