Effects of BMAL2 on Aerobic Glycolysis and Cell Proliferation in Acute Myeloid Leukemia Cells
10.19746/j.cnki.issn1009-2137.2024.02.012
- VernacularTitle:节律分子BMAL2对急性髓系白血病细胞有氧糖酵解和细胞增殖的影响
- Author:
Wei-Jing JIA
1
;
Jiao MU
;
Wen-Jing CUI
Author Information
1. 空军军医大学第一附属医院血液内科,陕西西安 710032
- Keywords:
basic helix-loop-helix ARNT like 2;
glucose metabolism;
cell proliferation;
acute myeloid leukemia
- From:
Journal of Experimental Hematology
2024;32(2):402-408
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of basic helix-loop-helix ARNT like 2(BMAL2)in acute myeloid leukemia(AML)patients and its correlation with prognosis,and analyze its effects on the aerobic glycolysis and proliferation of AML cells.Methods:The expressions of BMAL2 in bone marrow mononuclear cells(BMMCs)of AML patients and normal control group were detected by RT-qPCR.The correlation of BMAL2 expression with prognosis of AML patients was analyzed using public database of National Center for Biotechnology Information(NCBI).The interfering in BMAL2 expression of HL-60 and Kasumi-1 cells was performed using lentiviral vector-mediated shRNA.Cell glucose metabolism and proliferation were detected by using glucose uptake experiment,lactate content test,CCK-8 assay and cell colony formation test.Results:The expression level of BMAL2 mRNA in BMMCs of AML patients was significantly higher than normal control group(P＜0.01).The overall survival time of AML patients with high expression of BMAL2 was significantly shorter than those with low expression of BMAL2(P＜0.05).Knockdown of BMAL2 significantly reduced glucose uptake and lactate production in AML cell line HL-60 and Kasumi-1 cells.The results of RT-PCR and Western blot showed that BMAL2 promoted aerobic glycolysis by enhancing the expression of HIF1A in AML cells,thereby promoting cell proliferation.Conclusion:BMAL2 is highly expressed in AML patients,and promotes aerobic glycolysis by enhancing the expression of HIF1A,thereby promoting cell proliferation.
