The Effect of TCP1 Expression on the Proliferation and the Accu-mulation of Intracellular Drug of HL60/A and HL60 Cell and Its Mechanism
10.19746/j.cnki.issn1009-2137.2024.01.012
- VernacularTitle:TCP1表达对HL60和HL60/A细胞增殖及细胞内药物蓄积的调控作用及其机制
- Author:
Xiao-Fang CHEN
1
;
Xian-Ling CHEN
;
Yuan-Zhong CHEN
Author Information
1. 福建医科大学附属协和医院感染科
- Keywords:
TCP1;
acute myeloid leukemia;
intracellular drug accumulation;
MRP1;
P-gP;
PI3K/AKT signaling pathway
- From:
Journal of Experimental Hematology
2024;32(1):71-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of TCP1 expression on the proliferation and the accumulation of intracellular drug of HL60/A and HL60 cells and its possible molecular mechanism.Methods:Lentiviral transfection technology was used to construct HL60/A and HL60 cells with knocked down or overexpressed TCP1 and their control cells.The efficiency of knockdown and overexpression was evaluated by Western blot.The cell proliferation was detected by CCK-8 assay.The intracellular drug accumulation was detected by laser confocal detection and flow cytometry.The expression levels of MRP1,P-gP and p-AKT were evaluated by flow cytometry and Western blot.Results:After TCP1 was knocked down,the proliferation ability of HL60/A cells was significantly reduced,the accumulation of intracellular drug was significantly increased and the expression of MRP1 and P-gP protein were decreased.After TCP1 was overexpressed,the proliferation ability of HL60 was significantly increased,the accumulation of intracellular drug was significantly decreased and the expression of MRP1 and P-gP protein were increased.Intervention of LY294002 significantly antagonized the promotion on cell proliferation,the inhibition on intracellular drug accumulation and the expression of MRP1 and P-gP mediated by TCP1 overexpressing in HL60 cells.Conclusion:TCP1 can promote cell proliferation,improve the expression of MRP1 and P-gP by activating PI3K/AKT signal,and reduce intracellular drug accumulation.
