Inflammatory response in peste des petits ruminants virus infected LDG-2 cells is mediated by TLR2/MyD88/NF-κB signaling pathway
10.16303/j.cnki.1005-4545.2024.10.17
- VernacularTitle:小反刍兽疫病毒通过TLR2/MyD88/NF-κB信号通路介导LGD-2细胞炎症反应
- Author:
Tiantian SUN
1
;
Yun MU
;
Yongsheng KUANG
;
Shuyi YUAN
;
Shaohong CHEN
;
Fucheng GUO
;
You LIU
Author Information
1. 广东海洋大学滨海农业学院,广东湛江 524088
- Keywords:
peste des petits ruminants virus(PPRV);
TLR2 receptor;
MyD88/NF-κB signaling path-way;
inflammatory response
- From:
Chinese Journal of Veterinary Science
2024;44(10):2204-2212,2308
- CountryChina
- Language:Chinese
-
Abstract:
MTT assay,indirect immunofluorescence assay(IFA)and real-time fluorescence quanti-tative PCR(qRT-PCR)were used to observe the proliferation of PPRV in goat kidney cells(LDG-2).Western blot and qRT-PCR were used to evaluate the effects of PPRV infection on the expres-sion levels of TLR2,MyD88,NF-κB signaling pathway-related factors and their downstream in-flammatory factors.The results indicated that the significantly decreased cell survival rate and ob-vious cytopathic effect were observed at 36 h post PPRV infection,and the mRNA expression level of PPRV-N gene was significantly up-regulated.At the same time,the expression levels of TLR2,MyD88,p-p65 and p-IκBα,the ratio of p-p65/p65 and p-IκBα/IκBα and the mRNA expression levels of downstream inflammatory factors TNFα,IL-1β,IL-4 and IL-10 were significantly increased.Mo-reover,the expression levels of PPRV-N mRNA,TLR2,MyD88,p-p65 and p-IκBα,the ratio of p-p65/p65 and p-IκBα/IκBα and the mRNA expression levels of downstream inflammatory factors IL-1β and IL-4 in PPRV-infected cells were significantly decreased in the presence of the inhibitor C29 of TLR2.These findings implied that the TLR2/MyD88/NF-κBα signaling pathway can be activated by PPRV infection,which is beneficial to the replication and spread of the virus.Blocking down the activation of TLR2 can inhibit MyD88/NF-κB signaling pathway and viral replication in PPRV-infected cells.
