Effect of BLV-miRNAs on milk lactoperoxidase
10.16303/j.cnki.1005-4545.2024.06.23
- VernacularTitle:BLV-miRNAs对牛乳过氧化物酶的影响
- Author:
Yandi WANG
1
;
Guichi LIU
;
Yuejie YANG
;
Jianfa WANG
;
Shuai LIAN
;
Rui WU
Author Information
1. 黑龙江八一农垦大学动物科技学院农业农村部东北寒区牛病防治重点实验室/黑龙江省牛病防制重点实验室,黑龙江大庆 163319
- Keywords:
bovine leukemia virus;
lactoperoxidase;
BLV-miRNAs;
bovine mammary epithelial cells
- From:
Chinese Journal of Veterinary Science
2024;44(6):1274-1279
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to investigate the effect of bovine leukemia virus encoded microRNAs(BLV-miRNAs)on lactoperoxidase(LPO)in bovine mammary epithelial cells(BMECs).Firstly,the LPO content in the milk of BLV-positive cows,categorized by different viral loads,was quanti-fied by enzyme-linked immunosorbent assay(ELISA).Subsequently,BMECs were cultured in vitro and infected with(1 MOI)full-length BLV and BLV lacking miRNAs(BLV-ΔmiRNAs).Va-rious analytical techniques,including fluorescent quantitative PCR and ELISA,were used to assess LPO expression levels in different BMEC cohorts.Ten BLV-miRNAs were computationally predic-ted to target LPO using software tools such as StarMir.Based on these predictions,transfections of BLV-miRNAs were carried out and preliminary verification of their effects on target genes were performed.The results showed that compared to that of BLV-negative counterparts,the LPO levels in the milk of BLV-positive cows,stratified by high and low viral load,decreased by 8.73％and 9.68％,respectively.Invitro experiments further corroborated these trends,revealing a significant increase(P＜0.05)in LPO expression within BMECs following the deletion of BLV-miRNAs compared to the group infected with full-length BLV-infected group.Computational target site pre-dictions implicated BLV-miR-B1-5p,B3-3p,and B4-5p in the collective regulation of the LPO gene.Transfection of BLV-miR-B1-5p into BMECs resulted in a significant downregulation of LPO gene expression(P＜0.05),with the effect intensifying proportionally with the transfection dose.Simi-larly,the transfection of BLV-miR-B4-3p into BMECs significantly reduced LPO gene expression(P＜0.05)without showing a dose-dependent behavior.In conclusion,this research indicates that BLV-miRNAs can suppress LPO expression in BMECs.
