Organ-specific antigens of Clonorchis sinensis.
10.3347/kjp.2004.42.4.169
- Author:
Shunyu LI
;
Byung Suk CHUNG
;
Min Ho CHOI
;
Sung Tae HONG
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Clonorchis sinensis;
organ-specific antigen;
immunoblot;
7-kDa antigen
- MeSH:
Animals;
Antigens, Helminth/*analysis;
Clonorchis sinensis/anatomy & histology/*immunology;
Female;
Humans;
Male;
Mice;
Mice, Inbred BALB C;
Rabbits;
Research Support, Non-U.S. Gov't
- From:The Korean Journal of Parasitology
2004;42(4):169-174
- CountryRepublic of Korea
- Language:English
-
Abstract:
This study was carried out to find out specific proteins from different organs of Clonorchis sinensis. Crude extract, organ-specific and excretory-secretory (ES) proteins were analyzed by immunoblot with infected human sera. The bands of 7- and 17-kDa were main component of intestinal fluid and ES protein and commonly found in all organspecific proteins. The 17-kDa protein was observed from ES antigen, intestinal fluid, eggs and sperms, 26- and 28- kDa proteins were from the uterus, vitellaria, and ovary, and 34-, 37-, 43- and 50-kDa proteins were mainly from the testis and sperms. Serum of mice immunized with sperms reacted to the 50-kDa protein by immunoblotting and immunohistochemical staining showed a positive reaction at the seminal receptacle and seminiferous tubule. The present results show that the 7-kDa protein is a common antigen of every part or organ of C. sinensis, but different organs express their specific antigenic protein bands.
