Systematic Analysis of Histone Post-translational Modifications in Macrophages Regulated by Quercetin Based on Super-SILAC
10.13865/j.cnki.cjbmb.2024.10.1245
- VernacularTitle:基于Super-SILAC定量技术系统解析槲皮素调控巨噬细胞的组蛋白翻译后修饰图谱
- Author:
Zhi-Qing LIU
1
;
Lin-Hui ZHAI
;
Jing JI
;
Min-Jia TAN
;
Ping LIU
Author Information
1. 江苏海洋大学药学院,江苏省海洋药物化合物筛选重点实验室,江苏,连云港222005
- Keywords:
quercetin (Que);
epigenetics;
histone post-translational modifications;
super stable isotope labeling by amino acids in cell culture (super-SILAC);
macrophages
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(11):1574-1584
- CountryChina
- Language:Chinese
-
Abstract:
Quercetin (Que) is a flavonoid compound widely distributed in nature with various biological activities.Its anti-inflammatory effect plays a crucial role in many diseases,closely related to its regula-tion of histone post-translational modifications.However,there have been no detailed reports on the anti-inflammatory effects of quercetin regulating histone post-translational modifications.In this study,we first investigated the effect of quercetin on the M1 macrophages polarization.The results showed that quercetin can significantly down-regulate the levels of interleukin-1β(IL-1β) and interleukin-6 (IL-6 ) in M1 macrophages.Next,we used the super stable isotope labeling by amino acids in cell culture (super-SI-LAC) method derived from SILAC technology based on mass spectrometry to systematically analyze the post-translational modification levels of histone in macrophages treated with quercetin.A total of 30 his-tone modification sites were quantified,of which 12 histone lysine acetylation marks were significantly downregulated and 4 lysine methylation sites were upregulated (fold change>1.2,P<0.05),and some sites were verified by Western blot (WB),which was consistent with the mass spectrometry results.In conclusion,a comprehensive analysis of quercetin on regulating macrophage histone modifications in this study provides reliable data references and new insights for studying its anti-inflammatory mechanism.
