LncRNA 606938 Inhibits Proliferation of Colorectal Cancer Cells
10.13865/j.cnki.cjbmb.2024.05.1459
- VernacularTitle:LncRNA 606938抑制结直肠癌细胞增殖
- Author:
Hai-Li WU
1
;
Bing DUAN
;
Jin-E DU
;
Jia-Jing MA
;
Zhuo-Yu LI
Author Information
1. 山西大学生命科学学院生物工程系,太原 030006;山西大学生物技术研究所生物工程系,化学生物学与分子工程教育部重点实验室生物工程系,太原 030006
- Keywords:
long non-coding RNAs 606938(lncRNA 606938);
TRIM33/β-catenin signal;
colorectal cancer(CRC);
cell proliferation;
cell apoptosis
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(7):964-975
- CountryChina
- Language:Chinese
-
Abstract:
In recent years,the application of long non-coding RNAs(lncRNAs)in the treatment of dis-eases has been widely concerned.LncRNAs have been proved to play a crucial role in tumor therapy.They usually act as oncogene or suppressor genes to regulate the occurrence and development of tumors.Previously,our group discovered a new lncRNA 606938 that can be used as a target in colorectal cancer through transcriptome analysis.However,its molecular mechanism in colorectal cancer is still unclear.In this study,RT-qPCR test shows that lncRNA 606938 is low expressed in colorectal cancer cell lines com-pared with normal colon epithelial cells.The clonogenicity was decreased by 97％and 54.5％in lncRNA 606938 overexpressed DLD-1 and SW620 cells,respectively.The results of flow cytometry assay showed that overexpression of lncRNA 606938 caused cell cycle arrest at G1 phase(They were prolonged by 50.5％and 63.9％,respectively)and promoted apoptosis of colorectal cancer cells,which was increased by 1.9％and 3.3％,respectively.Western blot results showed that overexpression of lncRNA 606938 down-regulated the expression of related oncogenes(β-catenin,c-Myc,cyclin D1,cyclin D2,cyclin D3,CDK 4,CDK 6)in colorectal cancer cells,and the expression of tumor suppressor genes(TRIM33,caspase 2,and actived caspase 3)was up-regulated.The above results were reversed after knockdown of lncRNA 606938.Mechanistically,it has been confirmed through RNA pulldown assay,RIP assay,and Rescue assay that lncRNA 606938 can target and promote TRIM33 expression,thereby promoting the degradation of β-catenin and blocking the expression of β-catenin and its downstream oncogenes such as c-Myc and cycline D1 to inhibit the progression of colorectal cancer.This study elucidates the molecular mechanism by which the lncRNA 606938 targets TRIM33/β-catenin signaling pathway,inhibits the pro-liferation and promotes the apoptosis of colorectal cancer cells.This study reveals the potential of lncRNA 606938 as a tumor suppressor gene,providing new target and strategies for the clinical treatment of color-ectal cancer.
